University of Strasbourg, ICube Laboratory, Strasbourg, France.
Beckman Laser Institute and Medical Clinic, Laser Microbeam and Medical Program, Irvine, California, United States.
J Biomed Opt. 2019 Mar;24(7):1-7. doi: 10.1117/1.JBO.24.7.071610.
Quantitative diffuse optical imaging has the potential to provide valuable functional information about tissue status, such as oxygen saturation or blood content to healthcare practitioners in real time. However, significant technical challenges have so far prevented such tools from being deployed in the clinic. Toward achieving this goal, prior research introduced methods based on spatial frequency domain imaging (SFDI) that allow real-time (within milliseconds) wide-field imaging of optical properties but at a single wavelength. However, for this technology to be useful to clinicians, images must be displayed in terms of metrics related to the physiological state of the tissue, hence interpretable to guide decision-making. For this purpose, recent developments introduced multispectral SFDI methods for rapid imaging of oxygenation parameters up to 16 frames per seconds (fps). We introduce real-time, wide-field, and quantitative blood parameters imaging using spatiotemporal modulation of light. Using this method, we are able to quantitatively obtain optical properties at 100 fps at two wavelengths (665 and 860 nm), and therefore oxygenation, oxyhemoglobin, and deoxyhemoglobin, using a single camera with, at most, 4.2% error in comparison with standard SFDI acquisitions.
定量漫射光学成像是一种有潜力的技术，能够实时为医疗保健从业者提供有关组织状态的有价值的功能信息，例如氧饱和度或血液含量。然而，到目前为止，重大的技术挑战阻止了这些工具在临床中得到部署。为了实现这一目标，先前的研究引入了基于空间频域成像（SFDI）的方法，这些方法允许在单个波长下实时（毫秒内）对光学性质进行宽场成像。然而，为了使这项技术对临床医生有用，图像必须以与组织生理状态相关的指标显示，以便进行解释以指导决策。为此，最近的发展引入了多光谱 SFDI 方法，用于以每秒 16 帧（fps）的速度快速成像氧合参数。我们介绍了使用光的时空调制进行实时、宽场和定量血液参数成像的方法。使用这种方法，我们能够以 100 fps 的速度在两个波长（665 和 860nm）定量获得光学特性，因此能够定量获得氧合、氧合血红蛋白和脱氧血红蛋白，与标准 SFDI 采集相比，误差最大为 4.2%。
