Gu Jianjun, Xu Feng, Dang Yuan, Bu Xingyao
Clin Lab. 2019 Mar 1;65(3). doi: 10.7754/Clin.Lab.2018.180817.
Long noncoding RNAs (lncRNAs) are a novel type of endogenous noncoding RNA that are involved in the regulation of gene expression and play important roles in several types of cancer. LncRNA001089 is an intergenic lncRNA associated with cancer progression and tumor occurrence; however, the role and function of LncRNA001089 in glioma remains unclear.
In this study, we determined the expression levels of LncRNA001089 in tissues of glioma patients and explored its effect on glioma cell metastasis and proliferation using U251 glioma cell lines. Quantitative real-time PCR (qRT-PCR) technology was used to verify the expression levels of LncRNA001089 in the 127 tissues of glioma patients. Functional studies of LncRNA001089 were performed in glioma cells, including a colony formation assay, evaluation of proliferation by CCK-8, evaluation of apoptosis by flow cytometry, and evaluation of migration and invasion in vitro by Transwell assays. Tumorigenesis was evaluated in vivo in nude mice.
LncRNA001089 was downregulated in glioma tissues, evaluated by qRT-PCR. Kaplan-Meier analysis indicated that the downregulation of LncRNA001089 expression was associated with poor prognoses in glioma patients. Multivariate analysis demonstrated that LncRNA001089 downregulation and WHO high-grade glioma were independent factors that both predicted poor outcomes for glioma patients. Cells with LncRNA001089 stable overexpression exhibited decreased capacities for proliferation, migration, and invasion in vitro, and restrained nude mouse tumorigenesis in vivo, but LncRNA001089 overexpression enhanced apoptosis.
Our data suggest that LncRNA001089 plays a critical role in the development of glioma and may function as a potential novel biomarker and/or a therapeutic target for treatment of glioma.
长链非编码RNA（lncRNAs）是一类新型的内源性非编码RNA，参与基因表达调控，在多种癌症中发挥重要作用。LncRNA001089是一种与癌症进展和肿瘤发生相关的基因间lncRNA；然而，LncRNA001089在胶质瘤中的作用和功能仍不清楚。
在本研究中，我们测定了胶质瘤患者组织中LncRNA001089的表达水平，并使用U251胶质瘤细胞系探讨了其对胶质瘤细胞转移和增殖的影响。采用定量实时PCR（qRT-PCR）技术验证LncRNA001089在127例胶质瘤患者组织中的表达水平。在胶质瘤细胞中进行LncRNA001089的功能研究，包括集落形成试验、CCK-8法评估增殖、流式细胞术评估凋亡以及Transwell试验评估体外迁移和侵袭。在裸鼠体内评估肿瘤发生情况。
通过qRT-PCR评估，LncRNA001089在胶质瘤组织中表达下调。Kaplan-Meier分析表明，LncRNA001089表达下调与胶质瘤患者预后不良相关。多因素分析表明，LncRNA001089下调和世界卫生组织高级别胶质瘤是预测胶质瘤患者不良结局的独立因素。LncRNA001089稳定过表达的细胞在体外表现出增殖、迁移和侵袭能力下降，在体内抑制裸鼠肿瘤发生，但LncRNA001089过表达增强凋亡。
我们的数据表明，LncRNA001089在胶质瘤发生发展中起关键作用，可能作为一种潜在的新型生物标志物和/或胶质瘤治疗的靶点。
