Vinson LaQuia A, Gilbert Patrick R, Sanders Brian J, Moser Elizabeth, Gregory Richard L
Assistant professor and an assistant program director, in the School of Dentistry, at Indiana University, Indianapolis, Ind., USA;, Email:
Resident, in the School of Dentistry, at Indiana University, Indianapolis, Ind., USA.
J Dent Child (Chic). 2018 Sep 15;85(3):120-124.
The purpose of this study was to investigate the inhibitory in vitro effects of silver diamine fluoride (SDF) with and without a saturated solution of potassium iodide (SSKI) on established biofilm. Fifty μl of an overnight culture (10 CFU per mL) in Tryptic Soy Broth (TSB) and three ml of fresh TSB supplemented with one percent sucrose (TSBS) were incubated for 24 hours to establish an biofilm in six-well tissue culture plates. Four treatments (SDF, SSKI, SDF plus SSKI, and untreated control) were used to disrupt the biofilm. The biofilm groups were each treated with reagent and washed; the biofilm was collected, diluted, and spiral-plated onto blood agar plates; and an automated counting machine was used to determine the bacterial colony forming units (CFU). The control had significantly more CFU than the SSKI, SDF, and SDF plus SSKI groups (<.0001). The SSKI group had significantly more CFU than the SDF and SDF plus SSKI groups (<.0001). The SDF group had significantly fewer CFU than the SDF plus SSKI group (=.02). The reduction from the control was more than seven-fold for SDF, four-fold for SDF plus SSKI, and two-fold for SSKI. SDF alone, SDF plus SSKI, and SSKI disrupted an established biofilm. SDF alone had the greatest overall disruption.
本研究的目的是调查含或不含饱和碘化钾溶液（SSKI）的氟化氨银（SDF）对已形成的生物膜的体外抑制作用。在胰蛋白胨大豆肉汤（TSB）中50μl过夜培养物（每毫升10 CFU）和3ml补充有1%蔗糖的新鲜TSB（TSBS）在六孔组织培养板中孵育24小时以形成生物膜。使用四种处理方法（SDF、SSKI、SDF加SSKI和未处理的对照）来破坏生物膜。生物膜组分别用试剂处理并冲洗；收集生物膜，稀释，并螺旋接种到血琼脂平板上；使用自动计数机确定细菌菌落形成单位（CFU）。对照组的CFU明显多于SSKI、SDF和SDF加SSKI组（<.0001）。SSKI组的CFU明显多于SDF和SDF加SSKI组（<.0001）。SDF组的CFU明显少于SDF加SSKI组（=.02）。与对照组相比，SDF的减少超过7倍，SDF加SSKI的减少为4倍，SSKI的减少为2倍。单独使用SDF、SDF加SSKI和SSKI均可破坏已形成的生物膜。单独使用SDF的总体破坏作用最大。
