Department of Cariology and Operative Dentistry, Division of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU).
Endowed Department of International Oral Health Science, Tsurumi University School of Dental Medicine.
Dent Mater J. 2021 Jul 31;40(4):911-917. doi: 10.4012/dmj.2020-341. Epub 2021 Mar 18.
Effects of silver diamine fluoride preparations (SDFs) on cariogenic biofilm formation on root dentin (RD) were investigated. Streptococcus mutans (S. mutans) biofilms were formed on bovine RD blocks coated with one of three the SDFs (38%-SDF, 3.8%-SDF and 35%-SDF+potassium-iodide; SDF+KI) and a non-coated Control which were quantified (spectrometric-measurement) and thickness measured (optical coherence tomography) after 20 h. Bacterial viability test (BacLight) and biofilm-morphometry (SEM) of 2 h biofilms were also performed. The amounts of biofilms (bacteria and water insoluble glucan) and the thickness of biofilm were minimum on 38%-SDF specimen; 3.8%-SDF and SDF+KI had significantly more than that, but had significantly less than Control (p<0.05). Most S. mutans cells found dead and morphology damaged by 38%-SDF. Some dead bacteria and remarkably damaged biofilms were observed in case of 3.8%-SDF and SDF+KI. Inhibition potential of 3.8%-SDF and SDF+KI on S. mutans biofilm formation is almost similar, although not equivalent to 38%-SDF.
研究了银胺氟化物制剂（SDF）对牙本质（RD）致龋生物膜形成的影响。在涂有三种 SDF 之一（38%-SDF、3.8%-SDF 和 35%-SDF+碘化钾；SDF+KI）和未涂层对照的牛 RD 块上形成变形链球菌（S. mutans）生物膜，经过 20 小时后用分光光度法（spectrometric-measurement）定量和光学相干断层扫描（optical coherence tomography）测量厚度。还进行了 2 小时生物膜的细菌活力测试（BacLight）和生物膜形态计量学（SEM）。在 38%-SDF 标本上，生物膜的数量（细菌和水不溶性葡聚糖）和生物膜的厚度最小；3.8%-SDF 和 SDF+KI 的含量显著高于 38%-SDF，但显著低于对照（p<0.05）。大多数 S. mutans 细胞死亡，形态被 38%-SDF 破坏。在 3.8%-SDF 和 SDF+KI 的情况下，观察到一些死亡细菌和明显受损的生物膜。3.8%-SDF 和 SDF+KI 对 S. mutans 生物膜形成的抑制潜力几乎相似，尽管不如 38%-SDF。
