BIOS - Microdevices for Chemical Analysis group, MESA+ Institute for Nanotechnology, Techmed Centre, University of Twente, Hallenweg 15, 7522 NH Enschede, The Netherlands.
Department of Human Genetics, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands.
Lab Chip. 2019 Apr 9;19(8):1332-1343. doi: 10.1039/c8lc01137k.
Measuring biomolecule concentrations in the brain of living animals, in real time, is a challenging task, especially when detailed information at high temporal resolution is also required. Traditionally, microdialysis probes are used that generally have sampling areas in the order of about 1 mm2, and provide information on concentrations with a temporal resolution of at least several minutes. In this paper, we present a novel miniaturized push-pull perfusion sampling probe that uses an array of small 3 μm-wide sampling channels to sample neurotransmitters at a typical recovery rate of 61%, with a reduced risk of clogging. The added feature to segment the dialysate inside the probe into small water-in-decane droplets enables the detection of concentrations with a temporal resolution of a few seconds. Here we used the probe for in vivo recordings of neurotransmitter glutamate released upon electrical stimulation in the brain of a mouse to demonstrate the feasibility of the probe for real-time neurochemical brain analysis.
在活体动物的大脑中实时测量生物分子浓度是一项具有挑战性的任务，特别是当需要高时间分辨率的详细信息时。传统上，使用微透析探针，其采样面积通常在约 1mm2 左右，并以至少几分钟的时间分辨率提供浓度信息。在本文中，我们提出了一种新颖的小型推挽式灌注采样探针，该探针使用阵列的小 3μm 宽采样通道以 61%的典型恢复率采集神经递质，堵塞风险降低。在探针内将透析液分割成小的水在癸烷液滴的附加特征使能够以几秒钟的时间分辨率检测浓度。在这里，我们使用该探针在小鼠大脑中电刺激时释放的神经递质谷氨酸的体内记录来证明该探针用于实时神经化学脑分析的可行性。
