基于新型 DNA 水凝胶扩增信号探针与 DNA walker 扩增的 miRNA 多功能荧光检测
Versatile fluorescence detection of microRNA based on novel DNA hydrogel-amplified signal probes coupled with DNA walker amplification.
机构信息
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China.
出版信息
Chem Commun (Camb). 2019 Apr 7;55(27):3919-3922. doi: 10.1039/c9cc00565j. Epub 2019 Mar 14.
A novel DNA hydrogel-amplified versatile fluorescence platform combined with hybridization chain reaction (HCR) and DNA walking multiple amplification was developed for ultrasensitive detection of miRNA. The DNA hydrogel was loaded with large amounts of SYBR Green (SG) I dyes or CdTe quantum dots (QDs) to assemble versatile signal probes.
一种新型的 DNA 水凝胶扩增的多功能荧光平台，结合杂交链式反应 (HCR) 和 DNA 行走多重扩增，用于超灵敏检测 miRNA。DNA 水凝胶负载大量的 SYBR Green (SG) I 染料或 CdTe 量子点 (QDs)，以组装多功能信号探针。
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