Dwyer S D, Meyers K M
Thromb Res. 1986 Apr 15;42(2):139-51. doi: 10.1016/0049-3848(86)90290-2.
Aggregation of platelets in heparin- and citrate-anticoagulated platelet-rich-plasma (PRP) from rats anesthetized with methoxyflurane (M), diethyl ether (E), acepromazine/ketamine (A/K), or sodium pentobarbital (P) is described, as are platelet counts. Platelet counts were highest in heparin- or citrate-PRP from E and A/K anesthetized rats. Collagen and arachidonic acid (AA) induced aggregation in heparin-PRP only, and ADP induced greater aggregation in heparin-PRP than in citrate-PRP. Differences between citrate-PRP and heparin-PRP are probably due to citrate inhibition of platelet aggregation, since addition of citrate to heparin-PRP decreased aggregation, while addition of heparin to citrate-PRP did not alter aggregation. Aggregation of hirudin-PRP was slightly less than heparin-PRP. Anesthetics affected rat platelet aggregation: the rank order of the maximal extent of ADP-induced aggregation in citrate-PRP was M greater than E = A/K greater than P, and that for AA and collagen in heparin-PRP was E = A/K greater than M = P. The correlation between the effect of the anesthetics and activation of the sympathoadrenal system is discussed. It appeared that of the commonly used anticoagulants and anesthetics, heparin and methoxyflurane had the least influence on rat platelet aggregation.
描述了用甲氧氟烷（M）、乙醚（E）、乙酰丙嗪/氯胺酮（A/K）或戊巴比妥钠（P）麻醉的大鼠的肝素化和枸橼酸化富血小板血浆（PRP）中血小板的聚集情况，以及血小板计数。E组和A/K组麻醉大鼠的肝素化或枸橼酸化PRP中的血小板计数最高。胶原蛋白和花生四烯酸（AA）仅在肝素化PRP中诱导聚集，ADP在肝素化PRP中诱导的聚集比在枸橼酸化PRP中更大。枸橼酸化PRP和肝素化PRP之间的差异可能是由于枸橼酸盐对血小板聚集的抑制作用，因为向肝素化PRP中添加枸橼酸盐会降低聚集，而向枸橼酸化PRP中添加肝素不会改变聚集。水蛭素化PRP的聚集略低于肝素化PRP。麻醉剂影响大鼠血小板聚集：枸橼酸化PRP中ADP诱导聚集的最大程度的排序为M大于E = A/K大于P，肝素化PRP中AA和胶原蛋白诱导聚集的最大程度的排序为E = A/K大于M = P。讨论了麻醉剂的作用与交感肾上腺系统激活之间的相关性。似乎在常用的抗凝剂和麻醉剂中，肝素和甲氧氟烷对大鼠血小板聚集的影响最小。
