Häggroth L, Mattsson C, Felding P, Nilsson I M
Thromb Res. 1986 Jun 1;42(5):585-94. doi: 10.1016/0049-3848(86)90337-3.
Using affinity chromatography on lysine Sepharose 4B, a fast-acting tissue plasminogen activator inhibitor (t-PAI) was partially purified from t-PAI-rich plasma from patients with recurrent DVT. Its inhibition of tissue plasminogen activator (t-PA) was demonstrated in functional assays and its reaction with 125I-t-PA was analyzed by autoradiography following SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). When the t-PAI was mixed with an equimolar concentration of t-PA at 37 degrees C, the half-life of free one-chain and two-chain 125I-t-PA was 1.8 and 0.8 min, respectively. The rate of complex formation between 125I-t-PA and t-PAI was similar both in patient plasma, pregnancy plasma and platelet lysates made from platelet-rich normal, patient and pregnancy plasma. The molecular weights of the complexes between t-PA and the inhibitors in patient plasma and in the different platelet lysates were identical, while that of the inhibitor complex formed in pregnancy plasma was found slightly higher by SDS-PAGE indicating that the pregnancy plasma t-PAI differs from the fast-acting t-PAI found in plasma from thrombotic patients and in platelet lysates.
利用赖氨酸琼脂糖凝胶4B亲和层析法，从复发性深静脉血栓形成患者富含组织型纤溶酶原激活物（t-PA）的血浆中部分纯化出一种快速作用的组织型纤溶酶原激活物抑制剂（t-PAI）。在功能试验中证实了其对组织型纤溶酶原激活物（t-PA）的抑制作用，并在十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）后通过放射自显影分析了其与125I-t-PA的反应。当t-PAI与等摩尔浓度的t-PA在37℃混合时，游离单链和双链125I-t-PA的半衰期分别为1.8分钟和0.8分钟。125I-t-PA与t-PAI之间的复合物形成速率在患者血浆、妊娠血浆以及由富含血小板的正常血浆、患者血浆和妊娠血浆制备的血小板裂解物中相似。患者血浆和不同血小板裂解物中t-PA与抑制剂之间复合物的分子量相同，而通过SDS-PAGE发现妊娠血浆中形成的抑制剂复合物分子量略高，这表明妊娠血浆t-PAI与血栓形成患者血浆和血小板裂解物中发现的快速作用t-PAI不同。
