Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seoungbuk-gu, Seoul 02841, Republic of Korea.
Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seoungbuk-gu, Seoul 02841, Republic of Korea.
Bioresour Technol. 2019 Jun;282:245-253. doi: 10.1016/j.biortech.2019.03.024. Epub 2019 Mar 7.
Current research aimed to increase mixotrophic biomass from various organic carbon sources by exploring best light conditions. Three substrates glucose, acetic acid and glycerol were studied for their effects on mixotrophic microalgae cultivation under four light conditions. Light irradiance exhibited variability in growth response and photosynthetic efficiency based on type of substrates used in mixotrophic growth. Each substrate showed variability in light requirements for their effective assimilations. From growth responses, glucose and acetic acid respectively exhibited heterotrophic and mixotrophic (better growth in light) natures. Continuous light-deficient condition was adequate for effective mixotrophic growth as well as energy saving for glucose. However, light-sufficient condition required for effective acetic acid supported mixotrophic growth. Mixotrophic benefits from glycerol and its uptake by Chlorella protothecoides was negligible in all light conditions. Investigation of heterotrophic biomass contribution by various substrates in overall mixotrophic yield, glucose offered maximum approx. 43% contribution.
当前的研究旨在通过探索最佳光照条件来提高混合营养生物量，从各种有机碳源中获得。本研究以三种底物葡萄糖、乙酸和甘油为研究对象，考察了它们在四种光照条件下对混合营养微藻培养的影响。根据混合营养生长中使用的底物类型，光照辐照度表现出不同的生长响应和光合效率。每种底物的有效同化都需要不同的光照要求。从生长响应来看，葡萄糖和乙酸分别表现出异养和混合营养（在光照下生长更好）的特性。持续的弱光条件足以实现有效的混合营养生长，同时也节省了葡萄糖的能量。然而，有效的乙酸支持混合营养生长需要充足的光照条件。在所有光照条件下，甘油及其被小球藻吸收的混合营养益处都可以忽略不计。在混合营养总产率中，各种底物的异养生物量贡献的研究表明，葡萄糖提供了最大约 43%的贡献。
