Pan-Utai Wanida, Pornpukdeewattana Soisuda, Inrung Wilasinee, Thurakit Theera, Srinophakun Penjit
Department of Applied Microbiology, Institute of Food Research and Product Development, Kasetsart University, Bangkok 10900, Thailand.
School of Food Industry, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Life (Basel). 2025 Mar 5;15(3):407. doi: 10.3390/life15030407.
Microalgae show promise as a biomass and bioproduct for applications in various industries. The cultivation of microalgae plays a crucial role in optimizing biomass yield and bioproduct accumulation. The provision of inorganic carbon substrates substantially enhances microalgal growth and lipid biosynthesis, resulting in marked increases in the production of biofuels and other bioproducts. This study examined biomass and lipid accumulation in IFRPD 1061 under inorganic stress conditions, previously unreported. IFRPD 1061 was subjected to varying conditions of inorganic carbon substrates, 1-3 g·L sodium carbonate and bicarbonate concentration, to enhance biomass and lipid accumulation. Optimal productivity levels were observed with sodium bicarbonate addition of 3 g·L and 1 g·L for biomass and lipids, resulting in productivities of 392.64 and 53.57 mg·L·d, respectively. Results underlined the effectiveness of sodium carbonate and bicarbonate as inorganic carbon sources for stimulating microalgal growth and enhancing the production of high-value products. The extraction of lipids from freeze-dried biomass of IFRPD 1061 demonstrated optimal yield using methanol/hexane solvents compared with the control experiments. Lipid extraction yields using methanol/hexane were 42.18% and 46.81% from oven-dried and freeze-dried biomass, respectively. Lipids extracted from oven-dried IFRPD 1061 using methanol/hexane/chloroform solvents indicated the potential of methanol/hexane as a solvent for lipid extraction from dry microalgal biomass using an ultrasonic-assisted technique. This study contributes valuable insights into maximizing biofuel and bioproduct production from microalgae, highlighting as a promising candidate for industrial applications.
微藻有望成为应用于各行业的生物质和生物产品。微藻的培养对于优化生物质产量和生物产品积累起着关键作用。无机碳底物的供应显著促进微藻生长和脂质生物合成，从而使生物燃料和其他生物产品的产量显著增加。本研究考察了IFRPD 1061在无机胁迫条件下的生物质和脂质积累情况，此前未见报道。对IFRPD 1061施加不同条件的无机碳底物，即碳酸钠和碳酸氢钠浓度为1 - 3 g·L，以提高生物质和脂质积累。添加3 g·L碳酸氢钠时生物质产量最佳，添加1 g·L碳酸氢钠时脂质产量最佳，生物质和脂质的生产率分别为392.64和53.57 mg·L·d。结果强调了碳酸钠和碳酸氢钠作为无机碳源刺激微藻生长和提高高价值产品产量的有效性。与对照实验相比，从IFRPD 1061冻干生物质中提取脂质时，使用甲醇/己烷溶剂的产量最佳。使用甲醇/己烷从烘干和冻干生物质中提取脂质的产率分别为42.18%和46.81%。使用甲醇/己烷/氯仿溶剂从烘干的IFRPD 1061中提取的脂质表明，甲醇/己烷作为一种溶剂，具有通过超声辅助技术从干燥微藻生物质中提取脂质的潜力。本研究为最大化微藻生物燃料和生物产品产量提供了有价值的见解，突出了其作为工业应用有前景的候选者的地位。
