Plasencia Javier, Banttari Ernest E
Former Graduate Research Assistant.
Professor, Department of Plant Pathology, University of Minnesota, St. Paul 55108.
Plant Dis. 1997 Jan;81(1):53-56. doi: 10.1094/PDIS.1997.81.1.53.
Vascular colonization by Verticillium dahliae of 14 potato (Solanum tuberosum) cultivars was evaluated in field trials by a culture plate method and an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA). Whereas the culture plate technique quantifies fungal propa-gules, the monoclonal antibody-based immunoassay detects the soluble antigen, providing an indicator of fungal biomass. Differences in vascular colonization were evident with both methods; thus, cultivars could be ranked as susceptible, intermediate, or resistant. Linear regression analysis of means of each cultivar showed correlation coefficients (r) ranging from -0.606 to -0.835. Spearman's rank correlation coefficient (r) ranged from -0.65 to -0.91. Estimation by IC-ELISA of vascular colonization by V. dahliae in potato is as accurate as that by the culture plate technique, and results can be obtained in 3 days with an approximately 50% reduction in costs of materials and reagents. This quantitative immunoassay can be applied in potato breeding programs to evaluate resistance to Verticillium wilt.
通过平板培养法和间接竞争酶联免疫吸附测定法（IC-ELISA），在田间试验中评估了14个马铃薯（Solanum tuberosum）品种被大丽轮枝菌的维管束定殖情况。平板培养技术用于量化真菌繁殖体，而基于单克隆抗体的免疫测定则检测可溶性抗原，从而提供真菌生物量的指标。两种方法均显示维管束定殖存在差异；因此，品种可分为易感、中等或抗性。对每个品种的平均值进行线性回归分析，相关系数（r）在-0.606至-0.835之间。斯皮尔曼等级相关系数（r）在-0.65至-0.91之间。用IC-ELISA法估计马铃薯中被大丽轮枝菌的维管束定殖情况与平板培养技术一样准确，且3天即可获得结果，材料和试剂成本约降低50%。这种定量免疫测定可应用于马铃薯育种计划，以评估对黄萎病的抗性。
