Dan H, Ali-Khan S T, Robb J
Department of Molecular Biology and Genetics.
Department of Horticultural Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Plant Dis. 2001 Jul;85(7):700-705. doi: 10.1094/PDIS.2001.85.7.700.
A potato breeding population was evaluated for the presence of tolerance and resistance to Verticillium dahliae. Clones were ranked as susceptible or moderately and highly resistant based on symptom expression, and a species-specific polymerase chain reaction (PCR)-based assay was used to determine the Verticillium biomass in the bases of stems. A clone was designated as tolerant if the amount of fungus present in the host tissue was equal to or greater than the collective average amount for all clones in the symptom category above. Tolerant and resistant clones were identified in all trials, but the expression of tolerance was usually unstable across runs. The fungus was detected in some symptomless plants; on the other hand, some symptomatic plants were found to be pathogen-free. These observations emphasize the necessity of accurate quantification of the pathogen biomass in potato during Verticillium resistance breeding programs.
对一个马铃薯育种群体进行了评估，以确定其对大丽轮枝菌的耐受性和抗性。根据症状表现，将克隆分为易感、中度抗性和高度抗性，并用基于物种特异性聚合酶链反应（PCR）的检测方法来测定茎基部的大丽轮枝菌生物量。如果宿主组织中存在的真菌量等于或大于上述症状类别中所有克隆的总体平均量，则将该克隆指定为耐受性克隆。在所有试验中都鉴定出了耐受性和抗性克隆，但耐受性的表达在不同试验中通常不稳定。在一些无症状植物中检测到了这种真菌；另一方面，发现一些有症状的植物没有病原体。这些观察结果强调了在大丽轮枝菌抗性育种计划中准确量化马铃薯中病原体生物量的必要性。
