State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Int J Mol Sci. 2019 Mar 12;20(5):1232. doi: 10.3390/ijms20051232.
The rat is an important model animal used frequently in biological researches exploring the correlations between gut microbiome and a wide array of diseases. In this study, we used an extended ancestral-state reconstruction algorithm to predict the functional capabilities of the rat gastrointestinal microbiome. Our results indicate an apparent tendency toward metabolic heterogeneity along the longitudinal and transverse axes of the rat gastrointestinal tract (GIT). This heterogeneity was suggested by the enriched small-molecule transport activity and amino acid metabolism in the upper GIT, the aerobic energy metabolism in the stomach and the mucolysis-related metabolism in the lower GIT mucus layer. In contrast to prior results, many functional overlaps were observed when the gastrointestinal microbiomes of different hosts were compared. These overlaps implied that although both the biogeographic location and host genotype were prominent driving forces in shaping the gastrointestinal microbiota, the microbiome functions were similar across hosts when observed under similar physicochemical conditions at identical anatomical sites. Our work effectively complements the rat microbial biogeography dataset we released in 2017 and, thus, contributes to a better understanding and prediction of disease-related alterations in microbial community function.
大鼠是一种重要的模式动物，常用于探索肠道微生物组与广泛疾病之间的相关性的生物学研究。在这项研究中，我们使用了一种扩展的祖先状态重建算法来预测大鼠胃肠道微生物组的功能能力。我们的结果表明，大鼠胃肠道（GIT）的纵向和横向轴上存在明显的代谢异质性趋势。这种异质性表现在上 GIT 中丰富的小分子转运活性和氨基酸代谢、胃中的需氧能量代谢以及下 GIT 黏液层中的黏液溶解相关代谢。与先前的结果相比，当比较不同宿主的胃肠道微生物组时，观察到许多功能重叠。这些重叠表明，尽管生物地理位置和宿主基因型都是塑造胃肠道微生物群的突出驱动力，但在相同解剖部位观察到相似的理化条件下，宿主之间的微生物组功能相似。我们的工作有效地补充了我们在 2017 年发布的大鼠微生物生物地理学数据集，从而有助于更好地理解和预测与疾病相关的微生物群落功能改变。
