Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033, Japan; Laboratory of Geriatric Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033, Japan.
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033, Japan.
J Pharmacol Sci. 2019 Apr;139(4):346-351. doi: 10.1016/j.jphs.2019.02.006. Epub 2019 Feb 27.
Bridging accumulating insights from microscopic and macroscopic studies in neuroscience research requires monitoring of neuronal population dynamics and quantifying specific molecules or genes from the brain of identical animals. To this end, by minimizing the size and weight of an electrode array, we developed a method that records local field potential signals of multiple brain regions from one side of the hemisphere in a freely moving rodent. At the same time, extracellular cerebrospinal fluid for biochemical assays or a small part of brain tissue samples for gene expression assays are collected from the other side of the hemisphere. This method allows ongoing stable recordings and sample collections for at least two months. The methodological concept is applicable to a wide range of biological reactions at various spatiotemporal scales, allowing us to integrate an idea of physiolomics into existing omics analyses, leading to a new combination of multi-omics approaches.
为了将神经科学研究中微观和宏观研究的积累的见解联系起来，需要监测神经元群体的动态，并从相同动物的大脑中定量特定的分子或基因。为此，通过最小化电极阵列的大小和重量，我们开发了一种方法，该方法可从自由移动的啮齿动物的大脑半球的一侧记录多个脑区的局部场电位信号。同时，从大脑半球的另一侧收集用于生化分析的细胞外脑脊液或用于基因表达分析的脑组织样本的一小部分。该方法允许至少两个月的持续稳定记录和样本收集。该方法适用于各种时空尺度的广泛的生物学反应，使我们能够将生理组学的概念整合到现有的组学分析中，从而将多种组学方法的新组合。
