MRC-Laboratory for Molecular Cell Biology, University College London, London, WC1E 6BT, UK.
Department of Cell and Developmental Biology, University College London, London, WC1E 6BT, UK.
Nat Commun. 2019 Mar 15;10(1):1223. doi: 10.1038/s41467-019-09231-9.
Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments.
结合和复用显微镜方法对于理解细胞事件至关重要，但需要精心设计的工作流程。在这里，我们提出了一种稳健的、开源的方法，用于在自动化序列中处理、标记和成像活细胞或固定细胞。NanoJ-Fluidics 基于低成本乐高硬件，由基于 ImageJ 的软件控制，使得高内涵、多模式成像易于在任何显微镜上实现，具有很高的重现性。我们在事件驱动的、超分辨率活细胞到固定细胞和复用 STORM/DNA-PAINT 实验中展示了它的功能。
