Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 830011, Urumqi, Xinjiang, China.
College of Traditional Uyghur Medicine, Xinjiang Medical University, 830011, Urumqi, Xinjiang, China.
Sci Rep. 2019 Mar 15;9(1):4628. doi: 10.1038/s41598-019-41006-6.
We used Box-Behnken design-based (BBD) response surface methodology (RSM) in this research to optimize the extraction process of Traditional medicine Majun Mupakhi Ela (MME) and evaluate its effect on hydrocortisone-induced kidney yang deficiency. Three independent parameters were applied to evaluate the maximum phosphodiesterase type 5 (PDE5) inhibition activity of MME extracts in vitro. The optimal processing conditions (extraction time 2 h, solid-liquid ratio 1:16, extraction once) gave a maximum PDE5 inhibition rate of 84.10%, flavonoid content of 0.49 mg/ml, icariin content of 0.028 mg/ml and targeted extraction yield of 26.50%. In animal experiments, MME extracts significantly increased the adrenal mass index, semen weight index, preputial gland weight index, and penis weight index in mice; in the middle and high dose group, the level of serum testosterone increased by 7664.29% and 14207.14% respectively, compared with the model group, and the level of PDE5 decreased by 67.22% and 74.69% respectively compared with the control group. These results indicate that MME has a significant positive effect on the hypothalamus-pituitary-gonadal axis, improve mating ability and not only has inhibits PDE5 activity but also significantly inhibits the expression of PDE5 in penile tissues, potential to become erectile dysfunction (ED) therapies for the clinical management of patients with kidney yang deficiency.
我们在这项研究中使用基于 Box-Behnken 设计的（BBD）响应面法（RSM）来优化传统医学马骏木帕赫埃拉（MME）的提取工艺，并评估其对氢化可的松诱导的肾阳虚的影响。三个独立参数用于评估 MME 提取物在体外对最大磷酸二酯酶 5（PDE5）抑制活性的影响。最佳处理条件（提取时间 2 小时，固液比 1:16，提取一次）使 PDE5 抑制率达到 84.10%，黄酮含量为 0.49mg/ml，淫羊藿苷含量为 0.028mg/ml，目标提取收率为 26.50%。在动物实验中，MME 提取物显著增加了小鼠肾上腺质量指数、精液重量指数、包皮腺重量指数和阴茎重量指数；中、高剂量组血清睾酮水平分别比模型组提高了 7664.29%和 14207.14%，比对照组分别降低了 67.22%和 74.69%。这些结果表明，MME 对下丘脑-垂体-性腺轴有显著的正向作用，改善交配能力，不仅具有抑制 PDE5 活性的作用，而且还能显著抑制阴茎组织中 PDE5 的表达，有望成为肾阳虚患者临床治疗勃起功能障碍（ED）的治疗方法。
