Opt Lett. 2019 Mar 15;44(6):1383-1386. doi: 10.1364/OL.44.001383.
Raman spectroscopy provides molecular finger-printing of biological tissues. To achieve high spatial resolution, confocal Raman spectroscopy (micro-Raman) has been developed. To guide where micro-Raman is acquired, imaging guidance is necessary, especially for high scattering biological tissue. Reflectance confocal microscopy (RCM) has been integrated with micro-Raman. However, existing systems do not allow point-of-interest micro-Raman measurement with simultaneous RCM guidance. Here we demonstrate how a single laser can be used to localize and acquire micro-Raman signals within tissue with simultaneous real-time RCM imaging. Applications of this RCM-guided micro-Raman system for ex vivo samples and in vivo skin measurements are presented.
拉曼光谱提供了生物组织的分子指纹图谱。为了实现高空间分辨率，已经开发出共焦拉曼光谱（微拉曼）。为了指导微拉曼的获取位置，需要成像引导，特别是对于高散射的生物组织。反射共焦显微镜（RCM）已与微拉曼集成。然而，现有的系统不允许在实时 RCM 引导下进行具有实时 RCM 引导的微拉曼测量。在这里，我们展示了如何使用单个激光在组织内定位和获取微拉曼信号，同时进行实时 RCM 成像。介绍了这种 RCM 引导的微拉曼系统在离体样本和体内皮肤测量中的应用。
