从古代骨骼和牙齿中提取高度降解的DNA
Extraction of Highly Degraded DNA from Ancient Bones and Teeth.
作者信息
Dabney Jesse, Meyer Matthias
机构信息
Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
出版信息
Methods Mol Biol. 2019;1963:25-29. doi: 10.1007/978-1-4939-9176-1_4.
We provide a DNA extraction protocol optimized for the recovery of highly fragmented molecules preserved within bones and teeth. In this method, the hard tissue matrix is degraded using an EDTA/Proteinase K lysis buffer, and the DNA is purified using spin columns with silica membranes. This method efficiently recovers molecules as short as 35 base-pairs long.
我们提供了一种经过优化的DNA提取方案，用于从骨骼和牙齿中回收高度碎片化的分子。在该方法中，使用EDTA/蛋白酶K裂解缓冲液降解硬组织基质，并使用带有硅胶膜的离心柱纯化DNA。该方法能够有效地回收短至35个碱基对的分子。
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