从角蛋白和几丁质中提取DNA。
DNA Extraction from Keratin and Chitin.
作者信息
Campos Paula F, Gilbert M Thomas P
机构信息
Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark.
出版信息
Methods Mol Biol. 2019;1963:57-63. doi: 10.1007/978-1-4939-9176-1_7.
PMID:30875044
Abstract
DNA extracted from keratinous and chitinous materials can be a useful source of genetic information. To effectively liberate the DNA from these materials, buffers containing relatively high levels of DTT, proteinase K, and detergent are recommended, followed by purification using either silica column or organic methods.
摘要
从角质和几丁质材料中提取的DNA可以成为遗传信息的有用来源。为了有效地从这些材料中释放DNA，建议使用含有相对高水平二硫苏糖醇、蛋白酶K和去污剂的缓冲液，随后使用硅胶柱或有机方法进行纯化。
Zotero 插件