用于古代及其他降解样本的双链文库制备

Double-Stranded Library Preparation for Ancient and Other Degraded Samples.

作者信息

Henneberger Kirstin, Barlow Axel, Paijmans Johanna L A

机构信息

Institute for Biochemistry and Biology, University of Potsdam, Potsdam, Germany.

出版信息

Methods Mol Biol. 2019;1963:65-73. doi: 10.1007/978-1-4939-9176-1_8.

DOI:10.1007/978-1-4939-9176-1_8

PMID:30875045

Abstract

High-throughput sequencing (HTS) allows fast and cost-efficient sequencing of ancient DNA (aDNA) without prior information about what sequences should be targeted. One necessary step for HTS is the preparation of a sequencing library. Commercial kits are available for this purpose, but many of these are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency.

摘要

高通量测序（HTS）能够在无需预先了解应靶向何种序列的情况下，快速且经济高效地对古DNA（aDNA）进行测序。HTS的一个必要步骤是制备测序文库。为此有商业化试剂盒可供使用，但其中许多并不适用于aDNA或其他类型的受损DNA。在此，我们概述了一种针对古DNA进行优化的HTS文库制备方案。我们报告了一系列输入模板和接头浓度下的文库转化率。我们的结果表明该方案具有高效性。