Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland.
Int J Mol Sci. 2019 Mar 15;20(6):1309. doi: 10.3390/ijms20061309.
Oxidation of methionine to methionine sulfoxide is a type of posttranslational modification reversed by methionine sulfoxide reductases (Msrs), which present an exceptionally high number of gene copies in plants. The side-form general antioxidant function-specific role of each Msr isoform has not been fully studied. Thirty homologous genes of Msr type A (MsrA) and type B (MsrB) that originate from the genomes of , and were analyzed in silico. From 109 to 201 transcription factors and responsive elements were predicted for each gene. Among the species, 220 and 190 common transcription factors and responsive elements were detected for the and isoforms, respectively. In a comparison of 14 and 16 genes, 424 transcription factors and responsive elements were reported in both types of genes, with almost ten times fewer unique elements. The transcription factors mainly comprised plant growth and development regulators, transcription factors important in stress responses with significant overrepresentation of the myeloblastosis viral oncogene homolog (MYB) and no apical meristem, Arabidopsis transcription activation factor and cup-shaped cotyledon (NAC) families and responsive elements sensitive to ethylene, jasmonate, sugar, and prolamine. Gene Ontology term-based functional classification revealed that cellular, metabolic, and developmental process terms and the response to stimulus term dominated in the biological process category. Available experimental transcriptomic and proteomic data, in combination with a set of predictions, gave coherent results validating this research. Thus, new manners gene expression regulation, as well as new putative roles of Msrs, are proposed.
甲硫氨酸氧化为甲硫氨酸亚砜是一种翻译后修饰，可被甲硫氨酸亚砜还原酶（Msr）逆转，该酶在植物中具有极高数量的基因拷贝。每种 Msr 同工型的侧式一般抗氧化功能-特异性作用尚未得到充分研究。通过计算机分析了来自 、 和 基因组的 Msr 型 A（MsrA）和型 B（MsrB）的 30 个同源基因。对于每个基因，预测了从 109 到 201 个转录因子和响应元件。在这三个物种中，分别检测到 220 和 190 个共同的转录因子和响应元件，用于 和 同工型。在 14 个 和 16 个基因的比较中，在两种类型的基因中均报告了 424 个转录因子和响应元件，其独特元素的数量少了近十倍。转录因子主要包括植物生长和发育调节剂、在应激反应中重要的转录因子，其中髓样白血病病毒癌基因同源物（MYB）和无顶端分生组织、拟南芥转录激活因子和杯状子叶（NAC）家族的过表达，以及对乙烯、茉莉酸、糖和醇溶蛋白敏感的响应元件。基于基因本体论术语的功能分类表明，细胞、代谢和发育过程术语以及对刺激的响应术语在生物学过程类别中占主导地位。现有的实验转录组学和蛋白质组学数据，结合一组预测，给出了一致的结果，验证了这一研究。因此，提出了新的 基因表达调控方式以及 Msrs 的新潜在作用。
