Goerttler K, Loehrke H
Carcinogenesis. 1986 Jul;7(7):1187-9. doi: 10.1093/carcin/7.7.1187.
7,8-Benzoflavone (BF) was applied orally via stomach tube in doses of 50, 100, 200 and 400 mg/kg body weight to pregnant NMRI mice on the 18th day of gestation. BF application was followed 1 h later by oral administration of 60 mg/kg body weight dimethylbenz[a]anthracene (DMBA). As a rule this dose of DMBA does not lead to prenatal secondary effects and is not carcinogenic to either the mother animals or the F1 generation. Subsequent promotion of the F1 generation with the tumour promoter 12-O-tetradecanoylphorbol-13-acetate over a period of 12 weeks resulted in a high yield of skin papillomas and lung adenomas when no BF had been applied. With prior application of BF, the tumour yield could be significantly reduced.
在妊娠第18天，通过胃管给怀孕的NMRI小鼠口服7,8-苯并黄酮（BF），剂量分别为50、100、200和400毫克/千克体重。1小时后，口服给予60毫克/千克体重的二甲基苯并[a]蒽（DMBA）。通常，这个剂量的DMBA不会导致产前继发性影响，对母鼠或F1代也没有致癌性。随后，在12周的时间里，用肿瘤促进剂12-O-十四酰佛波醇-13-乙酸酯对F1代进行促进，结果在未应用BF的情况下，皮肤乳头状瘤和肺腺瘤的发生率很高。预先应用BF后，肿瘤发生率可显著降低。
