Department of Chemistry 'Ugo Schiff', University of Florence, Via della Lastruccia 3-13, 50019 Sesto Fiorentino, Italy.
Department of Chemistry 'Ugo Schiff', University of Florence, Via della Lastruccia 3-13, 50019 Sesto Fiorentino, Italy.
Talanta. 2019 Jun 1;198:15-22. doi: 10.1016/j.talanta.2019.01.095. Epub 2019 Jan 28.
We report a facile, low-cost and safe analytical method for estimation of total protein content, even in complex matrix like human serum, by exploiting the competition between proteins and polydopamine (PDA) for surface binding. The surface coating has been examined by using a microplate reader taking the advantage of the PDA absorbance in the visible region, obtaining new insights into the modelling of polydopamine deposition and polymer/protein adsorption competition. This is helpful for rational development of imprinted biosensors, and potentially offering a with broad applications ranging from diagnostic tools in medicine to food analysis. The isothermal adsorption of polydopamine on polystyrene surface of multi-well plate displays a Langmuir-shaped curve. It allows the determination of the parameters of polymer film formation useful for any analytical assay depending on the surface coating. Among these, the molecular imprinting and the optical and acoustic evanescent sensing.
我们报告了一种简便、低成本且安全的分析方法，用于估计总蛋白质含量，即使在像人血清这样复杂的基质中也是如此，该方法利用蛋白质和聚多巴胺（PDA）之间的表面结合竞争。通过利用 PDA 在可见区域的吸收，使用微孔板读取器对表面涂层进行了检查，从而深入了解了聚多巴胺沉积和聚合物/蛋白质吸附竞争的建模。这有助于合理开发印迹生物传感器，并有可能提供广泛的应用，从医学诊断工具到食品分析。聚多巴胺在多微孔板聚苯乙烯表面上的等温吸附呈现出Langmuir 型曲线。它允许确定对于任何依赖于表面涂层的分析测定有用的聚合物膜形成参数。其中包括分子印迹和光学和声学消逝波传感。
