Department of Chemistry, Bharathiar University, Coimbatore 641046, Tamil Nadu, India; BU-DRDO CLS, Bharathiar University Campus, Coimbatore 641046, Tamil Nadu, India.
Department of Chemistry, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
Talanta. 2019 Jun 1;198:249-256. doi: 10.1016/j.talanta.2019.01.114. Epub 2019 Feb 1.
A new simple pyrene based schiff base chemosensor 1 (nicotinic acid pyren-1-ylmethylene-hydrazide) has been constructed and is prepared from 1-pyrenecarboxaldehyde and nicotinic hydrazide. Notably, the chemosensor 1 exhibited remarkable colour changes while in the presence of trivalent metal ions like Bi & Al ion in DMSO-HO, (1:1 v/v, HEPES = 50 mM, pH = 7.4). The UV-Vis spectral investigation of chemosensor 1 showed that the maximum absorption peak appeared at 378 nm. In emission studies, chemosensor 1 develops weak fluorescence, while upon the addition of Bi and Al ions, it exhibits an enhancement of fluorescence intensity. Nevertheless, rest of metal ions have no changes in the emission spectra. The association constant of chemosensor 1 for binding to Bi & Al system had a value of 1.27 × 10 M and 1.53 × 10 M. The detection limits were 0.12 µM for Bi and 0.17 µM for Al respectively. The overall results reveal that chemosensor 1 can act as a dual-channel, highly selective, and sensitive probe for Bi and Al ions. Moreover, the fluorescence imaging of chemosensor 1 was applied in RAW 264.7 cell line and cytotoxicity assay prove that this chemosensor 1 is non-toxic as well as highly biocompatible.
一种新的基于吡咯的席夫碱化学传感器 1（烟酰基吡咯-1-亚甲基-腙）已经构建，由 1-吡咯甲醛和烟酰肼制备而成。值得注意的是，化学传感器 1 在 DMSO-HO（1:1 v/v，HEPES=50 mM，pH=7.4）存在三价金属离子如 Bi 和 Al 离子时表现出显著的颜色变化。化学传感器 1 的紫外可见光谱研究表明，最大吸收峰出现在 378nm。在荧光研究中，化学传感器 1 产生微弱的荧光，而当加入 Bi 和 Al 离子时，其荧光强度增强。然而，其余金属离子在发射光谱中没有变化。化学传感器 1 与 Bi 和 Al 体系结合的结合常数分别为 1.27×10 M 和 1.53×10 M。检测限分别为 0.12µM 的 Bi 和 0.17µM 的 Al。总体结果表明，化学传感器 1 可以作为一种双模式、高选择性和灵敏的 Bi 和 Al 离子探针。此外，化学传感器 1 的荧光成像应用于 RAW 264.7 细胞系，细胞毒性试验证明该化学传感器 1 不仅无毒，而且具有良好的生物相容性。
