Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang 330022, China.
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang 330022, China.
Talanta. 2019 Jun 1;198:316-322. doi: 10.1016/j.talanta.2019.02.006. Epub 2019 Feb 4.
A novel ratiometric fluorescent sensor for superoxide radical (O) detection was developed based on Schiff base polymer (SBP)/rhodamine-B (SBP/RDB) nanocomposites. The SBP/RDB was investigated by atomic force microscopy, scanning electron microscopy, transmission electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, N adsorption/desorption isotherms and fluorescence spectroscopy, etc. The results showed the SBP was two-dimensional (2D) crystalline nanosheet with the thickness of 0.5 nm. RDB could be self-assembled on each of 2D SBP surface to form SBP/RDB due to the hydrogen bond between N atoms of SBP and -COOH of RDB as well as π-π interaction. The SBP/RDB exhibited the characteristic fluorescence emission of both SBP at 485 nm and RDB at 585 nm under an excitation of 270 nm. The 2D SBP was not only acted as template to assemble RDB, but also used as reference fluorescence for O detection. The SBP/RDB fluorescence probe for O detection demonstrated high sensitivity and selectivity with good linear range (11.1 ± 0.76 nM-8.0 ± 0.36 μM) and low detection limit (3.7 ± 0.42 nM). Because of the low cost and simple operation, the work sheds some new light to construct ratiometric fluorescent sensors based on SBP to detect O in drinking water and human blood serum for commercial applications.
基于席夫碱聚合物（SBP）/罗丹明 B（SBP/RDB）纳米复合材料，开发了一种用于超氧自由基（O）检测的新型比率荧光传感器。通过原子力显微镜、扫描电子显微镜、透射电子显微镜、X 射线粉末衍射、傅里叶变换红外光谱、N 吸附/解吸等温线和荧光光谱等手段对 SBP/RDB 进行了研究。结果表明，SBP 为二维（2D）结晶纳米片，厚度为 0.5nm。RDB 可以通过 SBP 中的 N 原子与 RDB 的-COOH 之间的氢键以及π-π 相互作用自组装到每个 2D SBP 表面上，形成 SBP/RDB。SBP/RDB 在 270nm 激发下，表现出 SBP 在 485nm 和 RDB 在 585nm 的特征荧光发射。2D SBP 不仅用作组装 RDB 的模板，还用作 O 检测的参考荧光。O 检测的 SBP/RDB 荧光探针具有高灵敏度和选择性，线性范围良好（11.1±0.76nM-8.0±0.36μM），检测限低（3.7±0.42nM）。由于成本低、操作简单，这项工作为基于 SBP 构建用于饮用水和人血清中 O 检测的比率荧光传感器提供了一些新的思路，具有商业应用价值。
