基于金纳米粒子-牛血清白蛋白@铽/鸟苷酸纳米级配位聚合物的超氧阴离子比率荧光检测

Ratiometric fluorescence detection of superoxide anion based on AuNPs-BSA@Tb/GMP nanoscale coordination polymers.

作者信息

Liu Nan, Hao Juan, Cai Keying, Zeng Mulan, Huang Zhenzhong, Chen Lili, Peng Bingxian, Li Ping, Wang Li, Song Yonghai

机构信息

Key Laboratory of Functional Small Organic Molecule, Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, P. R. China.

出版信息

Luminescence. 2018 Feb;33(1):119-124. doi: 10.1002/bio.3380. Epub 2017 Aug 3.

DOI:10.1002/bio.3380

PMID:28776941

Abstract

A novel ratiometric fluorescence nanosensor for superoxide anion (O ) detection was designed with gold nanoparticles-bovine serum albumin (AuNPs-BSA)@terbium/guanosine monophosphate disodium (Tb/GMP) nanoscale coordination polymers (NCPs) (AuNPs-BSA@Tb/GMP NCPs). The abundant hydroxyl and amino groups of AuNPs-BSA acted as binding points for the self-assembly of Tb and GMP to form core-shell AuNPs-BSA@Tb/GMP NCP nanosensors. The obtained probe exhibited the characteristic fluorescence emission of both AuNPs-BSA and Tb/GMP NCPs. The AuNPs-BSA not only acted as a template to accelerate the growth of Tb/GMP NCPs, but also could be used as the reference fluorescence for the detection of O . The resulting AuNPs-BSA@Tb/GMP NCP ratiometric fluorescence nanosensor for the detection of O demonstrated high sensitivity and selectivity with a wide linear response range (14 nM-10 μM) and a low detection limit (4.7 nM).

摘要

一种用于检测超氧阴离子（O）的新型比率荧光纳米传感器，是用金纳米颗粒-牛血清白蛋白（AuNPs-BSA）@铽/鸟苷单磷酸二钠（Tb/GMP）纳米级配位聚合物（NCPs）（AuNPs-BSA@Tb/GMP NCPs）设计而成。AuNPs-BSA丰富的羟基和氨基作为Tb和GMP自组装形成核壳型AuNPs-BSA@Tb/GMP NCP纳米传感器的结合点。所获得的探针呈现出AuNPs-BSA和Tb/GMP NCPs两者的特征荧光发射。AuNPs-BSA不仅作为模板加速Tb/GMP NCPs的生长，还可作为检测O的参考荧光。所得用于检测O的AuNPs-BSA@Tb/GMP NCP比率荧光纳米传感器具有高灵敏度和选择性，线性响应范围宽（14 nM-10 μM），检测限低（4.7 nM）。