Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193, PR China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193, PR China.
Talanta. 2019 Jun 1;198:8-14. doi: 10.1016/j.talanta.2019.01.082. Epub 2019 Jan 25.
Organophosphorus pesticides (OPs) are frequently for pest control in the agriculture industry. Accumulation of OPs is harmful to the environment and human health. Thus, facile and portable detection of organophosphorus pesticides is of great importance. Among these methods, the fluorescence assay holds the advantages of high sensitivity, simplicity, nondestructive properties. Conventional fluorophores have the drawbacks of poor photostability and low signal-to-noise ratio due to their aggregation-caused quenching drawbacks at high concentration or in the aggregate state. Aggregation-induced emission fluorogens (AIEgens) are one key to develop next-generation fluorescence sensor due to their high emission efficiency in the aggregated state. 1,2-bis[4-(3-sulfonatopropoxyl) phenyl]-1,2-diphenylethene (BSPOTPE) is a typical AIE molecule containing two hydroxyl group. In this study, a fluorescence sensor based on BSPOTPE-SiO-MnO sandwich nanocomposites was fabricated. Thiocholine (TCh), which produced from acetylthiocholine(ATCh) by the hydrolysis of acetylcholinesterase (AChE), can "turn on" the fluorescence sensor. Based on the inhibition effect of OPs on AChE activity and the corresponding "turn off" effect on the fluorescence sensor, an AIE-based assay for OPs determination was developed. The fabricated sensor for paraoxon determination has a good linear relationship in the range of 1-100 μg/L and the LOD of 1 μg/L. Moreover, a simple, convenient fluorescence strip for visual semi-quantitative of OPs was fabricated, indicating this "on-off" fluorescent sensor is promising for on-site and infield detection.
有机磷农药（OPs）常用于农业害虫防治。OPs 的积累对环境和人体健康有害。因此，方便、便携的 OPs 检测具有重要意义。在这些方法中，荧光分析具有高灵敏度、简单、无损的特点。由于在高浓度或聚集状态下会发生聚集猝灭，传统荧光团存在光稳定性差和信噪比低的缺点。聚集诱导发射荧光团（AIEgens）是开发下一代荧光传感器的关键之一，因为它们在聚集状态下具有高发射效率。1,2-双[4-（3-磺丙氧基）苯基]-1,2-二苯基乙烯（BSPOTPE）是一种典型的 AIE 分子，含有两个羟基。在这项研究中，制备了基于 BSPOTPE-SiO-MnO 夹层纳米复合材料的荧光传感器。硫代胆碱（TCh）是由乙酰胆碱酯酶（AChE）水解乙酰硫代胆碱（ATCh）产生的，可以“开启”荧光传感器。基于 OPs 对 AChE 活性的抑制作用及其对荧光传感器的相应“关闭”效应，开发了一种基于 AIE 的 OPs 测定方法。用于对氧磷测定的制备传感器在 1-100μg/L 的范围内具有良好的线性关系，LOD 为 1μg/L。此外，还制备了一种用于 OPs 可视化半定量的简单、方便的荧光条带，表明这种“开-关”荧光传感器有望用于现场和野外检测。
