Department of Chemistry, College of Science, Beijing Forestry University, Beijing, 100083, China.
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China.
Sci Rep. 2021 Feb 25;11(1):4655. doi: 10.1038/s41598-021-84221-w.
The development of detecting residual level of abamectin B in apples is of great importance to public health. Herein, we synthesized a octopus-like azobenzene fluorescent probe 1,3,5-tris (5'-[(E)-(p-phenoxyazo) diazenyl)] benzene-1,3-dicarboxylic acid) benzene (TPB) for preliminary detection of abamectin B in apples. The TPB molecule has been characterized by ultraviolet-visible absorption spectrometry, H-nuclear magnetic resonance, fourier-transform infrared (FT-IR), electrospray ionization mass spectroscopy (ESI-MS) and fluorescent spectra. A proper determination condition was optimized, with limit of detection and limit of quantification of 1.3 µg L and 4.4 μg L, respectively. The mechanism of this probe to identify abamectin B was illustrated in terms of undergoing aromatic nucleophilic substitution, by comparing fluorescence changes, FT-IR and ESI-MS. Furthermore, a facile quantitative detection of the residual abamectin B in apples was achieved. Good reproducibility was present based on relative standard deviation of 2.2%. Six carboxyl recognition sites, three azo groups and unique fluorescence signal towards abamectin B of this fluorescent probe demonstrated reasonable sensitivity, specificity and selectivity. The results indicate that the octopus-like azobenzene fluorescent probe can be expected to be reliable for evaluating abamectin B in agricultural foods.
检测苹果中阿维菌素 B 残留量的发展对公众健康具有重要意义。在此，我们合成了一种章鱼状的偶氮苯荧光探针 1,3,5-三(5'-[(E)-(对苯氧基偶氮)偶氮基]苯-1,3-二羧酸)苯(TPB)，用于初步检测苹果中的阿维菌素 B。TPB 分子已通过紫外可见吸收光谱、H 核磁共振、傅里叶变换红外(FT-IR)、电喷雾电离质谱(ESI-MS)和荧光光谱进行了表征。优化了适当的测定条件，检测限和定量限分别为 1.3 µg L 和 4.4 µg L。通过比较荧光变化、FT-IR 和 ESI-MS，说明了该探针识别阿维菌素 B 的机制是芳香亲核取代。此外，还实现了苹果中残留阿维菌素 B 的简便定量检测。基于相对标准偏差为 2.2%，显示出良好的重现性。该荧光探针对阿维菌素 B 具有 6 个羧基识别位点、3 个偶氮基团和独特的荧光信号，表现出合理的灵敏度、特异性和选择性。结果表明，这种章鱼状偶氮苯荧光探针有望可靠地用于评估农业食品中的阿维菌素 B。
