Department of Medical Microbiology & Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150, Kota Bharu, Kelantan, Malaysia.
Department of Medical Microbiology & Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150, Kota Bharu, Kelantan, Malaysia; School of Health Sciences, Universiti Sains Malaysia, Health Campus, 16150, Kubang Kerian, Kelantan, Malaysia.
Mol Cell Probes. 2019 Apr;44:63-68. doi: 10.1016/j.mcp.2019.03.001. Epub 2019 Mar 12.
This study highlighted the performance of the developed integrated loop-mediated isothermal amplification (LAMP) coupled with a colorimetric DNA-based magnetogenosensor. The biosensor operates through a DNA hybridization system in which a specific designed probe captures the target LAMP amplicons. We demonstrated the magnetogenosensor assay by detecting pathogenic Leptospira, which causes leptospirosis. The color change of the assay from brown to blue indicated a positive result, whereas a negative result was indicated by the assay maintaining its brown color. The DNA biosensor was able to detect DNA at a concentration as low as 200 fg/μl, which is equivalent to 80 genomes/reaction. The specificity of the biosensor assay was 100% when it was evaluated with 172 bacterial strains. An integrated LAMP and probe-specific magnetogenosensor was successfully developed, promising simple and rapid visual detection in clinical diagnostics and service as a point-of-care device.
本研究重点介绍了开发的集成环介导等温扩增（LAMP）与比色 DNA 磁传感器相结合的性能。该生物传感器通过 DNA 杂交系统进行操作，其中特定设计的探针捕获靶 LAMP 扩增子。我们通过检测引起钩端螺旋体病的致病性钩端螺旋体来演示磁传感器检测。检测结果为阳性时，检测从棕色变为蓝色；检测结果为阴性时，检测仍保持棕色。该 DNA 生物传感器能够检测低至 200 fg/μl 的 DNA，相当于 80 个基因组/反应。当用 172 种细菌菌株评估时，生物传感器检测的特异性为 100%。成功开发了一种集成的 LAMP 和探针特异性磁传感器，有望在临床诊断和服务中实现简单快速的可视化检测，并作为一种即时检测设备。
