College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China.
College of Chemistry and Material Sciences, South-Central University for Nationalities, Wuhan 430074, China.
Toxins (Basel). 2020 Sep 2;12(9):565. doi: 10.3390/toxins12090565.
Aflatoxin contamination in agricultural products has posed serious health hazards and brought huge economic loss in the food and feed industries. Monitoring aflatoxins in various foods and feeds has become a crucial means to protect public health. This study aimed to report an immuno-loop-mediated isothermal amplification (iLAMP) assay by using an anti-idiotypic nanobody-phage for on-site and rapid detection of aflatoxin in real samples. The iLAMP method was developed on the basis of a competitive immunoassay and LAMP reaction performed in a simple water bath. This method can provide visualized test results: violet color represents positive samples while sky blue represents negative. The visual detection limits of iLAMP for aflatoxin B, B, G, and G in peanut samples were 1.6, 1.6, 3.2, and 16 μg/kg, respectively. The developed assay was verified with high performance liquid chromatography (HPLC) for the analysis of aflatoxins in peanuts, which demonstrated that the iLAMP method can be applied to the detection of aflatoxin in real samples. The novel iLAMP assay eliminates the need for aflatoxin conjugates, the antibody labeling process, and special equipment, and offers an alternative to existing methods with advantages of time-saving, cost-effectiveness, and ease-of-use.
农产品中黄曲霉毒素的污染对食品和饲料工业造成了严重的健康危害和巨大的经济损失。监测各种食品和饲料中的黄曲霉毒素已成为保护公众健康的重要手段。本研究旨在报告一种基于抗独特型纳米抗体-噬菌体的免疫环介导等温扩增（iLAMP）检测方法，用于现场和快速检测实际样品中的黄曲霉毒素。iLAMP 方法是在简单的水浴中进行竞争性免疫分析和 LAMP 反应的基础上开发的。该方法可以提供可视化的检测结果：紫色表示阳性样品，天蓝色表示阴性样品。iLAMP 对花生样品中黄曲霉毒素 B1、B2、G1 和 G2 的可视检测限分别为 1.6、1.6、3.2 和 16μg/kg。该方法已通过高效液相色谱法（HPLC）对花生中黄曲霉毒素的分析进行了验证，表明 iLAMP 方法可用于实际样品中黄曲霉毒素的检测。该新型 iLAMP 检测方法消除了黄曲霉毒素缀合物、抗体标记过程和特殊设备的需求，与现有方法相比具有省时、经济高效和易于使用的优势。
