基于探针的共聚焦激光内镜术追踪活鼠体内 SKOV 细胞的荧光聚合物微球。
Fluorescent Polymer Dots for Tracking SKOV Cells in Living Mice with Probe-Based Confocal Laser Endomicroscopy.
机构信息
Shanghai Med-X Engineering Center for Medical Equipment and Technology, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China.
The Key Laboratory of Resource Chemistry of Ministry of Education, Shanghai Key Laboratory of Rare Earth Functional Materials, and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Shanghai Normal University, Shanghai, 200234, People's Republic of China.
出版信息
Mol Imaging Biol. 2019 Dec;21(6):1026-1033. doi: 10.1007/s11307-019-01343-4.
PURPOSE
Probe-based confocal laser endomicroscopy (pCLE) is a novel technique allowing real-time and high-resolution imaging in vivo. It provides microscopic images and increases the penetration depth of tissues compared with conventional white light endoscopy. The aim of the present study was to track ovarian cancer cells in organs by fluorescent polymer dots based on pCLE.
PROCEDURES
SKOV-mCherry cells were incubated with polymer dots for 24 h in a serum-free culture medium. Labeled cells were administrated to nude mice via intravenous, intraperitoneal, and lymph node injection. The fluorescent signals of labeled cells in organs were observed by pCLE. Furthermore, the results were confirmed by frozen section analysis.
RESULTS
pCLE displayed fluorescence signals of labeled cells in the vessels of organs. Besides, the accumulations of labeled cells visualized in detoxification organs like the spleen and kidney were increased with time.
CONCLUSIONS
In this article, we present a real-time and convenient method for tracking SKOV-mCherry in living mice by combined fluorescent polymer dots with pCLE.
目的
基于探针的共聚焦激光内镜检查（pCLE）是一种新型技术，可实现实时和高分辨率的体内成像。它提供微观图像并增加了组织的穿透深度，与传统的白光内镜检查相比。本研究的目的是通过基于 pCLE 的荧光聚合物点跟踪卵巢癌细胞在器官中的迁移。
方法
将 SKOV-mCherry 细胞在无血清培养基中与聚合物点孵育 24 小时。通过静脉内、腹腔内和淋巴结注射将标记的细胞施用于裸鼠。通过 pCLE 观察器官中标记细胞的荧光信号。此外，通过冷冻切片分析证实了结果。
结果
pCLE 显示了器官血管中标记细胞的荧光信号。此外，随时间推移，标记细胞在解毒器官如脾脏和肾脏中的积累增加。
结论
本文提出了一种实时、方便的方法，通过将荧光聚合物点与 pCLE 相结合，追踪活鼠体内的 SKOV-mCherry。
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