Departamento de Medicina Veterinária Preventiva (DMVP), Setor de Virologia (SV), Universidade Federal de Santa Maria (UFSM), Avenida Roraima, 1000, prédio 63A, Centro de Eventos, Santa Maria, RS, 97105-900, Brazil.
Braz J Microbiol. 2019 Apr;50(2):557-563. doi: 10.1007/s42770-019-00058-6. Epub 2019 Mar 15.
Bovine pestiviruses, e.g., bovine viral diarrhea virus types 1 (BVDV-1 or Pestivirus A), BVDV-2 (Pestivirus B), and HoBi-like pestiviruses (HoBiPeV or Pestivirus H), have been shown to circulate in Brazilian cattle in varied proportions. In this study, we identified genetically pestiviruses circulating in beef cattle in Rio Grande do Sul, the southern most Brazilian state. Screening of serum of 15.584 beef calves destined to be export by an antigen capture ELISA and, subsequently, by reverse-transcription polymerase chain reaction (RT-PCR), revealed 135 containing pestivirus RNA. Genetic typing of these viruses based on nucleotide sequencing and phylogenetic analysis of the 5' untranslated region (5' UTR) of the viral genome allowed for the identification of 90 different viruses, being 38 BVDV-1 (42.2%), 31 BVDV-2 (34.4%), and 21 HoBiPeV (23.4%). Among BVDV-1, only subtypes BVDV-1a (n = 28, 31.1%) and BVDV-1b (n = 10, 11.1%) were identified. All 31 BVDV-2 isolates belonged to BVDV-2b subtype and the 21 HoBiPeV viruses clustered to subgroup 3a. Thus, this study provides an approximate genetic profile of pestiviruses circulating in beef cattle in a traditional Brazilian beef cattle-raising state.
牛瘟病毒，例如牛病毒性腹泻病毒 1 型（BVDV-1 或瘟病毒 A）、BVDV-2（瘟病毒 B）和 HoBi 样瘟病毒（HoBiPeV 或瘟病毒 H），已被证明在巴西牛群中以不同比例传播。在这项研究中，我们鉴定了巴西南部南里奥格兰德州肉牛中循环的瘟病毒。通过抗原捕获 ELISA 对 15584 头出口肉牛的血清进行筛查，随后通过逆转录聚合酶链反应（RT-PCR），发现 135 头含有瘟病毒 RNA。基于病毒基因组 5'非翻译区（5'UTR）的核苷酸测序和系统进化分析对这些病毒进行遗传分型，确定了 90 种不同的病毒，其中 38 种为 BVDV-1（42.2%）、31 种为 BVDV-2（34.4%）和 21 种为 HoBiPeV（23.4%）。在 BVDV-1 中，仅鉴定出 BVDV-1a 亚型（n=28，31.1%）和 BVDV-1b 亚型（n=10，11.1%）。所有 31 株 BVDV-2 分离株均属于 BVDV-2b 亚型，21 株 HoBiPeV 病毒聚类到 3a 亚群。因此，本研究提供了巴西传统肉牛养殖州肉牛中循环的瘟病毒的近似遗传特征。
