Mósena Ana Cristina S, Weber Matheus N, Cibulski Samuel P, Silveira Simone, Silva Mariana S, Mayer Fabiana Q, Canal Cláudio W
Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves 9090, Porto Alegre, RS, CEP 91540-000, Brazil.
Laboratório de Biologia Molecular, Instituto de Pesquisas Veterinárias Desidério Finamor, Fundação Estadual de Pesquisa Agropecuária, Eldorado do Sul, RS, Brazil.
Arch Virol. 2017 Apr;162(4):1119-1123. doi: 10.1007/s00705-016-3199-7. Epub 2016 Dec 21.
Bovine viral diarrhea virus 1 (BVDV-1) belongs to the genus Pestivirus within the family Flaviviridae. Based on the 5' untranslated region (UTR) sequence, BVDV-1 can be divided into at least 17 subtypes (1a though 1q). BVDV-1i is an uncommon subtype that has been reported in the United Kingdom and Uruguay. Here, we report the complete genome sequence of the first subtype 1i BVDV-1 (strain ACM/BR/2016) isolated from cattle in southern Brazil. The genome is 12,231 nt in length and contains a single ORF that encodes a polyprotein of 3,896 amino acids, flanked by 5' and 3'UTRs of 325 and 220 nt, respectively. Phylogenetic inferences based on the whole genome, the 5'UTR, and the N region showed that strain ACM/BR/2016 is closely related to previously characterized BVDV-1i members. Its 5'UTR shares the highest nucleotide identity (90.5%) with BVDV-1i strains from United Kingdom, and its N is most closely related to that of a Uruguayan strain (90.6%). To the best of our knowledge, this is the first BVDV-1i strain from which the whole genome has been completely sequenced and characterized. The complete genome of a BVDV-1i will help future studies on pestivirus evolution and heterogeneity.
牛病毒性腹泻病毒1型（BVDV-1）属于黄病毒科瘟病毒属。根据5'非翻译区（UTR）序列，BVDV-1可至少分为17个亚型（1a至1q）。BVDV-1i是一种不常见的亚型，已在英国和乌拉圭有过报道。在此，我们报告了从巴西南部牛群中分离出的首例1i亚型BVDV-1（毒株ACM/BR/2016）的全基因组序列。该基因组长度为12231个核苷酸，包含一个单一开放阅读框，编码一个由3896个氨基酸组成的多蛋白，两侧分别是325个核苷酸的5'UTR和220个核苷酸的3'UTR。基于全基因组、5'UTR和N区域的系统发育推断表明，毒株ACM/BR/2016与先前鉴定的BVDV-1i成员密切相关。其5'UTR与来自英国的BVDV-1i毒株具有最高的核苷酸同一性（90.5%），其N区域与乌拉圭一株毒株的N区域关系最为密切（90.6%）。据我们所知，这是首个全基因组已被完全测序和鉴定的BVDV-1i毒株。BVDV-1i的全基因组将有助于未来对瘟病毒进化和异质性的研究。
