Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture, PR China, Jilin Academy of Agricultural Sciences, Changchun 130033, Jilin, PR China.
Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture, PR China, Jilin Academy of Agricultural Sciences, Changchun 130033, Jilin, PR China.
Microb Pathog. 2019 May;130:190-195. doi: 10.1016/j.micpath.2019.03.014. Epub 2019 Mar 13.
Beauveria bassiana (B. bassiana) is a broad-spectrum entomopathogenic species of fungi which is a natural enemy of Ostrinia furnacalis (O. furnacalis). Nevertheless, the precise mechanism of pathogenicity difference of B. bassiana strains on O. furnacalis has not been investigated before. In this study, two B. bassiana strains isolated from the infected O. furnacalis and exhibited different pathogenicity were chose to analyze the gene expression using RNA-sequencing analysis. To investigate the significantly differentially expressed genes (DEGs) of these two strains, total RNA was extracted and Cuffdiff software was applied to perform the significance analysis of the microarrays method. qRT-PCR was applied to verify the expression of DEGs. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analyses were applied to evaluate the functions of DEGs. Data showed 72 up-regulated and 192 down-regulated genes in hyper-pathogenic strain ZK193 in comparison with hypo-pathogenic strain ZK203. Genes involved in fungal growth, sporulation and toxin production were up-regulated in hyper-pathogenic strain ZK193. GO enrichment analysis of DEGS showed that the most observably enriched biological processes of regulated genes were the single-organism process, the metabolic process, the cellular process and biological regulation. KEGG enrichment pathway demonstrated that the almost enriched groups were metabolic pathways, such as lipid metabolism, transport and catabolism, amino acid metabolism, and carbohydrate metabolism. In conclusion, these results will help us to further understand the reason why different B. bassiana strains exhibit different pathogenicity on the same host, even under the same conditions. In addition, transcriptome data will provide a theoretical basis for exploring latent virulence factors in the future.
球孢白僵菌（B. bassiana）是一种广谱的昆虫病原真菌，是玉米螟（O. furnacalis）的天敌。然而，B. bassiana 菌株对玉米螟致病力差异的确切机制尚未得到研究。在本研究中，选择了两株从感染的玉米螟中分离出来的、表现出不同致病性的 B. bassiana 菌株，利用 RNA 测序分析进行基因表达分析。为了研究这两株菌株的显著差异表达基因（DEGs），提取总 RNA，并应用 Cuffdiff 软件对微阵列方法进行显著性分析。应用 qRT-PCR 验证 DEGs 的表达。应用基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路分析评估 DEGs 的功能。数据显示，与低致病性菌株 ZK203 相比，高致病性菌株 ZK193 中有 72 个上调和 192 个下调基因。参与真菌生长、孢子形成和毒素产生的基因在高致病性菌株 ZK193 中上调。DEGs 的 GO 富集分析表明，调控基因最显著富集的生物学过程是单个生物体过程、代谢过程、细胞过程和生物调节。KEGG 富集通路表明，几乎富集的组是代谢途径，如脂质代谢、运输和分解代谢、氨基酸代谢和碳水化合物代谢。总之，这些结果将帮助我们进一步了解为什么不同的 B. bassiana 菌株在同一宿主甚至在相同条件下表现出不同的致病性。此外，转录组数据将为未来探索潜在的毒力因子提供理论依据。
