Institute of Entomology, Provincial Key Laboratory for Agricultural Pest Management of Mountainous Regions, Guizhou University, Guiyang, 550025, People's Republic of China.
Guizhou Tobacco Science Research Institute, Guiyang, 550081, People's Republic of China.
Sci Rep. 2021 Mar 24;11(1):6797. doi: 10.1038/s41598-021-86269-0.
Ultraviolet A (UV-A) radiation is a significant environmental factor that causes photoreceptor damage, apoptosis, and oxidative stress in insects. Ostrinia furnacalis is an important pest of corn. To understand the adaptation mechanisms of insect response to UV-A exposure, this study revealed differentially expressed genes (DEGs) and differently expressed metabolites (DEMs) in O. furnacalis under UV-A exposure. Three complementary DNA libraries were constructed from O. furnacalis adult females (CK, UV1h, and UV2h), and 50,106 expressed genes were obtained through Illumina sequencing. Of these, 157 and 637 DEGs were detected in UV1h and UV2h after UV-A exposure for 1 and 2 h, respectively, compared to CK, with 103 and 444 upregulated and 54 and 193 downregulated genes, respectively. Forty four DEGs were detected in UV2h compared to UV1h. Comparative transcriptome analysis between UV-treated and control groups revealed signal transduction, detoxification and stress response, immune defense, and antioxidative system involvement. Metabolomics analysis showed that 181 (UV1h vs. CK), 111 (UV2h vs. CK), and 34 (UV2h vs. UV1h) DEMs were obtained in positive ion mode, while 135 (UV1h vs. CK), 93 (UV2h vs. CK), and 36 (UV2h vs. UV1h) DEMs were obtained in negative ion mode. Moreover, UV-A exposure disturbed amino acid, sugar, and lipid metabolism. These findings provide insight for further studies on how insects protect themselves under UV-A stress.
紫外线 A（UV-A）辐射是一种重要的环境因素，会导致昆虫的光感受器损伤、细胞凋亡和氧化应激。玉米螟是玉米的重要害虫。为了了解昆虫对 UV-A 暴露的适应机制，本研究揭示了 UV-A 暴露下玉米螟中差异表达基因（DEGs）和差异表达代谢物（DEMs）。从玉米螟成虫雌性（CK、UV1h 和 UV2h）构建了三个 cDNA 文库，并通过 Illumina 测序获得了 50,106 个表达基因。其中，UV-A 暴露 1 和 2 小时后，UV1h 和 UV2h 中分别检测到 157 和 637 个 DEGs，与 CK 相比，分别有 103 和 444 个上调基因和 54 和 193 个下调基因。UV2h 与 UV1h 相比，检测到 44 个 DEGs。UV 处理组和对照组之间的比较转录组分析表明，信号转导、解毒和应激反应、免疫防御和抗氧化系统参与其中。代谢组学分析显示，正离子模式下获得了 181 个（UV1h vs. CK）、111 个（UV2h vs. CK）和 34 个（UV2h vs. UV1h）DEMs，负离子模式下获得了 135 个（UV1h vs. CK）、93 个（UV2h vs. CK）和 36 个（UV2h vs. UV1h）DEMs。此外，UV-A 暴露扰乱了氨基酸、糖和脂质代谢。这些发现为进一步研究昆虫在 UV-A 胁迫下如何自我保护提供了线索。
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