Universidad Tecnológica Nacional (UTN), Facultad Regional de Haédo, París 532, 1706 Haedo, Buenos Aires, Argentina.
Instituto de Investigaciones para la Industria Química (INIQUI), CONICET-UNSa, Argentina; Facultad de Ciencias Naturales, UNSa, Salta, Argentina.
Ecotoxicol Environ Saf. 2019 Jul 15;175:19-28. doi: 10.1016/j.ecoenv.2019.02.071. Epub 2019 Mar 15.
In the present study the polycyclic aromatic hydrocarbon removal and metabolic adaptation of Amycolatopsis tucumanensis DSM 45259 were investigated. Analysis of one-dimensional gel electrophoresis of crude cell extracts revealed differential synthesis of proteins which were identified by MALDI-TOF. To elucidate the phenanthrene metabolic pathway in A. tucumanensis DSM45259, two-dimensional electrophoresis and detection of phenanthrene degradation intermediates by GS-MS were performed. The presence of aromatic substrates resulted in changes in the abundance of proteins involved in the metabolism of aromatic compounds, oxidative stress response, energy production and protein synthesis. The obtained results allowed us to clarify the phenanthrene catabolic pathway, by confirming the roles of several proteins involved in the degradation process and comprehensive adaptation. This may clear the way for more efficient engineering of bacteria in the direction of more effective bioremediation applications.
本研究旨在探讨 Amycolatopsis tucumanensis DSM 45259 对多环芳烃的去除和代谢适应能力。通过对粗细胞提取物的一维凝胶电泳分析，揭示了差异合成的蛋白质，并通过 MALDI-TOF 进行了鉴定。为了阐明 A. tucumanensis DSM45259 中菲的代谢途径，进行了二维电泳和 GS-MS 检测菲降解中间产物。芳香底物的存在导致参与芳香化合物代谢、氧化应激响应、能量产生和蛋白质合成的蛋白质丰度发生变化。获得的结果允许我们通过确认降解过程中涉及的几种蛋白质的作用以及全面的适应来阐明菲的分解途径。这可能为朝着更有效的生物修复应用方向更有效地工程细菌铺平道路。
