Guerrero Daiana S, Bertani Romina P, Ledesma Ana, Frías M de Los Angeles, Romero Cintia M, Dávila Costa José S
Planta Piloto de Procesos Industriales Microbiológicos- (PROIMI-CONICET), Av. Belgrano y Pasaje Caseros, T4001 MVB, Tucumán, Argentina.
Estación Experimental Agroindustrial Obispo Colombres - Sección Fitopatología, Av. William Cross 3150, T4101 XAC, Tucumán, Argentina.
Heliyon. 2022 May 20;8(5):e09472. doi: 10.1016/j.heliyon.2022.e09472. eCollection 2022 May.
The production of bioethanol and sugar from sugarcane is an important economic activity in several countries. Sugarcane is susceptible to different phytopathogens. Over the last years, the red stripe disease caused by the bacterium subsp. produced significant losses in sugarcane crops. Bio-nanotechnology emerged as an eco-friendly alternative to the biosynthesis of antimicrobial molecules. The aims of this study were to (a) produce extracellular silver nanoparticles using the heavy metal resistant strain , (b) evaluate their antibacterial effect and (c) determine the potential of silver nanoparticles to protect sugarcane against red stripe disease. synthesized spherical silver nanoparticles with an average size of 35 nm. Nanoparticles were able to control the growth of subsp. in assays. In addition, assays in sugarcane showed a control upon the red stripe disease when silver nanoparticles were applied as preventive treatment. The Disease Severity Index was 28.94% when silver nanoparticles were applied 3 days before inoculation with subsp. . To our knowledge, this is the first report of silver nanoparticles extracellularly synthesized by an strain that were able to inhibited the growth of subsp. and control the red stripe disease in sugarcane.
利用甘蔗生产生物乙醇和糖是多个国家的一项重要经济活动。甘蔗易受不同植物病原体的侵害。在过去几年中，由细菌亚种引起的红条病给甘蔗作物造成了重大损失。生物纳米技术作为一种生态友好型的抗菌分子生物合成替代方法应运而生。本研究的目的是：（a）使用重金属抗性菌株生产细胞外银纳米颗粒；（b）评估其抗菌效果；（c）确定银纳米颗粒保护甘蔗免受红条病侵害的潜力。合成了平均尺寸为35纳米的球形银纳米颗粒。纳米颗粒能够在试验中控制亚种的生长。此外，在甘蔗上进行的试验表明，当将银纳米颗粒作为预防性处理应用时，对红条病有防治作用。在接种亚种前3天施用银纳米颗粒时，病情严重指数为28.94%。据我们所知，这是首次报道由菌株细胞外合成的银纳米颗粒能够抑制亚种的生长并控制甘蔗中的红条病。
