Callaway C, Falcon C, Grant G, Maurer D H, Auerbach A D, Rosenwaks Z, Pollack M S
Hum Immunol. 1986 Jun;16(2):200-4. doi: 10.1016/0198-8859(86)90048-0.
Like fetal fibroblasts and amniotic fluid cells, cultured chorionic villus cells can also be HLA typed with selected typing sera after preincubation with gamma interferon to promote better antigen expression. A modified procedure now in use would also allow any of these cell types to be tested for the presence or absence of all known HLA A,B,C, and DR antigens with standard preplated typing trays. This procedure was used to confirm that an on-going pregnancy had resulted from the successful in vitro fertilization and implantation of an anonymous donor's ovum and could also be of major use in rape or artificial insemination cases when the identity of the possible father(s) is not known.
与胎儿成纤维细胞和羊水细胞一样，培养的绒毛膜绒毛细胞在与γ干扰素预孵育以促进更好的抗原表达后，也可以用选定的分型血清进行HLA分型。目前使用的一种改良方法还可以使用标准预铺板分型托盘对这些细胞类型中的任何一种进行所有已知HLA A、B、C和DR抗原的有无检测。该方法用于确认持续妊娠是由匿名捐赠者卵子的体外受精和成功植入所致，在强奸或人工授精案件中，当可能的父亲身份不明时，该方法也可能有重要用途。
