de Lorenzo V, Neilands J B
J Bacteriol. 1986 Jul;167(1):350-5. doi: 10.1128/jb.167.1.350-355.1986.
A cloned 8.3-kilobase-pair DNA fragment carrying all the genes (iucABCD iutA) of the aerobactin iron transport system of plasmid pColV-K30 was subjected to in vitro mutagenesis to afford mutant genes iucA, iucC, and iucA iucC. Complementation analyses and identification of aerobactin precursors accumulated by Escherichia coli cells harboring the different constructions allowed assignment of the iucA and iucC genes to discrete steps in biosynthesis of the siderophore from N epsilon-acetyl-N epsilon-hydroxylysine and citrate. Plasmid pVLN10, a derivative carrying a DNA fragment complementing an iucC mutation, expressed in a minicell system a single 62,000-dalton protein as the product of this gene.
携带质粒pColV-K30气杆菌素铁转运系统所有基因（iucABCD iutA）的一个克隆的8.3千碱基对DNA片段接受了体外诱变，以产生突变基因iucA、iucC以及iucA iucC。通过对含有不同构建体的大肠杆菌细胞积累的气杆菌素前体进行互补分析和鉴定，可将iucA和iucC基因分配到从N-ε-乙酰-N-ε-羟基赖氨酸和柠檬酸盐生物合成铁载体的不同步骤中。质粒pVLN10是携带可互补iucC突变的DNA片段的衍生物，在一个小细胞系统中表达出一种单一的62,000道尔顿蛋白质，作为该基因的产物。
