绿脓菌素DF13/气杆菌素受体蛋白的亚克隆及参与高铁气杆菌素摄取的pColV-K30决定多肽的鉴定。
Subcloning of the cloacin DF13/aerobactin receptor protein and identification of a pColV-K30-determined polypeptide involved in ferric-aerobactin uptake.
作者信息
Krone W J, Luirink J, Koningstein G, Oudega B, de Graaf F K
出版信息
J Bacteriol. 1983 Nov;156(2):945-8. doi: 10.1128/jb.156.2.945-948.1983.
Abstract
A plasmid containing a pColV-K30 fragment that encoded only for the cloacin DF13/aerobactin receptor protein was constructed. Escherichia coli cells harboring this plasmid were sensitive to cloacin DF13 but were unable to take up ferric-aerobactin. Another pColV-K30-determined polypeptide (molecular weight, 50,000), localized in the membrane fraction, was essential for the uptake of ferric-aerobactin.
摘要
构建了一个含有仅编码阴沟肠毒素DF13/气杆菌素受体蛋白的pColV-K30片段的质粒。携带该质粒的大肠杆菌细胞对阴沟肠毒素DF13敏感，但无法摄取高铁气杆菌素。另一种定位于膜组分的由pColV-K30决定的多肽（分子量为50,000）对于高铁气杆菌素的摄取至关重要。
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