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Adenosine diphosphate ribosylation of dinitrogenase reductase and adenylylation of glutamine synthetase control ammonia excretion in ethylenediamine-resistant mutants of Azospirillum brasilense Sp7.在巴西固氮螺菌Sp7的乙二胺抗性突变体中,固氮酶还原酶的二磷酸腺苷核糖基化作用以及谷氨酰胺合成酶的腺苷酰化作用控制着氨的排泄。
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Assimilation of 13NH4+ by Azospirillum brasilense grown under nitrogen limitation and excess.巴西固氮螺菌在氮限制和氮过量条件下对13NH4+的同化作用。
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7
Nucleotide sequence of the gene encoding the nitrogenase iron protein (nifH) of Azospirillum brasilense and identification of a region controlling nifH transcription.巴西固氮螺菌固氮酶铁蛋白(nifH)编码基因的核苷酸序列及nifH转录调控区域的鉴定。
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The product of glnL is not essential for regulation of bacterial nitrogen assimilation.谷氨酰胺合成酶基因(glnL)的产物对于细菌氮同化的调节并非必不可少。
J Bacteriol. 1983 Apr;154(1):516-9. doi: 10.1128/jb.154.1.516-519.1983.
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Cloning of the glnA, ntrB and ntrC genes of Klebsiella pneumoniae and studies of their role in regulation of the nitrogen fixation (nif) gene cluster.肺炎克雷伯菌谷氨酰胺合成酶基因(glnA)、氮调节蛋白B基因(ntrB)和氮调节蛋白C基因(ntrC)的克隆及其在固氮(nif)基因簇调控中的作用研究。
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Nitrogen regulatory locus "glnR" of enteric bacteria is composed of cistrons ntrB and ntrC: identification of their protein products.肠道细菌的氮调节基因座“glnR”由顺反子ntrB和ntrC组成:其蛋白质产物的鉴定。
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控制巴西固氮螺菌中固氮基因表达和组氨酸转运的调控突变

Regulatory mutation that controls nif expression and histidine transport in Azospirillum brasilense.

作者信息

Fischer M, Levy E, Geller T

出版信息

J Bacteriol. 1986 Jul;167(1):423-6. doi: 10.1128/jb.167.1.423-426.1986.

DOI:10.1128/jb.167.1.423-426.1986
PMID:3087965
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC212899/
Abstract

Mutagenesis of Azospirillum brasilense with nitrosoguanidine and selection on ethylenediamine yielded prototrophs which fixed nitrogen in the presence of ammonia. Nitrogenase activity in mutant strains exceeded that of the wild type three- to sixfold. The same mutants were also constitutive for histidine transport. Enzyme activities involved in ammonia assimilation were not affected by the mutation. The data suggest that the mutation occurred at a site which regulates nif and histidine transport functions.

摘要

用亚硝基胍对巴西固氮螺菌进行诱变,并在乙二胺上进行筛选,得到了在氨存在下能固定氮的原养型菌株。突变菌株中的固氮酶活性比野生型高出三到六倍。同样的突变体在组氨酸转运方面也是组成型的。参与氨同化的酶活性不受该突变的影响。数据表明,该突变发生在一个调节固氮基因和组氨酸转运功能的位点上。