控制巴西固氮螺菌中固氮基因表达和组氨酸转运的调控突变
Regulatory mutation that controls nif expression and histidine transport in Azospirillum brasilense.
作者信息
Fischer M, Levy E, Geller T
出版信息
J Bacteriol. 1986 Jul;167(1):423-6. doi: 10.1128/jb.167.1.423-426.1986.
Abstract
Mutagenesis of Azospirillum brasilense with nitrosoguanidine and selection on ethylenediamine yielded prototrophs which fixed nitrogen in the presence of ammonia. Nitrogenase activity in mutant strains exceeded that of the wild type three- to sixfold. The same mutants were also constitutive for histidine transport. Enzyme activities involved in ammonia assimilation were not affected by the mutation. The data suggest that the mutation occurred at a site which regulates nif and histidine transport functions.
摘要
用亚硝基胍对巴西固氮螺菌进行诱变，并在乙二胺上进行筛选，得到了在氨存在下能固定氮的原养型菌株。突变菌株中的固氮酶活性比野生型高出三到六倍。同样的突变体在组氨酸转运方面也是组成型的。参与氨同化的酶活性不受该突变的影响。数据表明，该突变发生在一个调节固氮基因和组氨酸转运功能的位点上。
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