Ankenbauer R, Hanne L F, Cox C D
J Bacteriol. 1986 Jul;167(1):7-11. doi: 10.1128/jb.167.1.7-11.1986.
Twelve mutants of Pseudomonas aeruginosa PAO defective in pyoverdin production were isolated (after chemical and transposon mutagenesis) that were nonfluorescent and unable to grow on medium containing 400 microM ethylenediaminedi(o-hydroxyphenylacetic acid). Four mutants were unable to produce hydroxamate, six were hydroxamate positive, one was temperature sensitive for pyoverdin production, and another was unable to synthesize pyoverdin on succinate minimal medium but was capable of synthesizing pyoverdin when grown on Casamino Acids medium (Difco Laboratories, Detroit, Mich.). The mutations were mapped on the PAO chromosome. All the mutations affecting pyoverdin production were located at 65 to 70 min, between catA1 and mtu-9002.
分离出了12株绿脓杆菌PAO的突变体（经化学诱变和转座子诱变后），这些突变体在绿脓菌素产生方面存在缺陷，它们无荧光，且无法在含有400微摩尔乙二胺二（邻羟基苯乙酸）的培养基上生长。4个突变体无法产生异羟肟酸，6个突变体异羟肟酸呈阳性，1个对绿脓菌素产生具有温度敏感性，另一个在琥珀酸基本培养基上无法合成绿脓菌素，但在酪蛋白氨基酸培养基（底特律密歇根州迪福科实验室）上生长时能够合成绿脓菌素。这些突变被定位在PAO染色体上。所有影响绿脓菌素产生的突变都位于65至70分钟处，在catA1和mtu - 9002之间。
