Höfte M, Dong Q, Kourambas S, Krishnapillai V, Sherratt D, Mergeay M
Laboratory of Phytopathology, University of Gent, Belgium.
Mol Microbiol. 1994 Dec;14(5):1011-20. doi: 10.1111/j.1365-2958.1994.tb01335.x.
Pyoverdin production by Pseudomonas aeruginosa strain 7NSK2 was induced by Zn(II) in the presence of iron. A mutant was isolated in which Zn(II) no longer induced pyoverdin production. The sss gene which was inactivated in this mutant was cloned and sequenced. Its protein sequence showed 50% identity to the XerC protein of Escherichia coli, which is a member of the lambda integrase family of site-specific recombinases. An open reading frame was found upstream of sss whose protein sequence showed strong identity to DapF, the diaminopimelate epimerase. In E. coli, xerC is part of a multicistronic unit that also contains dapF. The sss gene of P. aeruginosa could restore site-specific recombination at cer in an E. coli xerC mutant and the E. coli xerC gene could complement a genomic sss mutation in P. aeruginosa.
在铁存在的情况下，铜绿假单胞菌菌株7NSK2的绿脓菌素产生由锌（II）诱导。分离出一个突变体，其中锌（II）不再诱导绿脓菌素产生。对该突变体中失活的sss基因进行了克隆和测序。其蛋白质序列与大肠杆菌的XerC蛋白有50%的同一性，XerC蛋白是位点特异性重组酶的λ整合酶家族的成员。在sss上游发现了一个开放阅读框，其蛋白质序列与二氨基庚二酸差向异构酶DapF有很强的同一性。在大肠杆菌中，xerC是一个多顺反子单元的一部分，该单元也包含dapF。铜绿假单胞菌的sss基因可以在大肠杆菌xerC突变体中恢复cer处的位点特异性重组，而大肠杆菌的xerC基因可以补充铜绿假单胞菌基因组中的sss突变。
