Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, N-0315, Oslo, Norway.
Université Paris Est, ICMPE (UMR7182), CNRS, UPEC, F 94320, Thiais, France.
Carbohydr Polym. 2019 Jun 1;213:403-410. doi: 10.1016/j.carbpol.2019.01.106. Epub 2019 Jan 30.
Synthesis of novel host-guest functionalized polymers is presented along with structural characterization using small-angle X-ray scattering (SAXS) of the resulting nanoparticles. Mono-6-deoxy-mono-6-azidoβCD (NβCD) was grafted onto alkyne-functionalized pullulan via the "click" reaction copper(I)-catalyzed azide alkyne cycloaddition (CuAAC) and an adamantane-modified dextran was prepared via the same strategy. Characterization of the polymers was carried out using nuclear magnetic resonance (NMR) spectroscopy, gel filtration chromatography (GFC), isothermal titration calorimetry (ITC) and SAXS. Nanoparticles were created via host-guest interactions between the well-defined βCD-pullulans and adamantane-modified dextran. Characterization was carried out using dynamic light scattering (DLS) and SAXS, which revealed spherical particles in the sub-100 nm range. The studies shed light on the importance of molecular structure and host-guest ratio on crucial properties such as particle size, size distribution, porosity and stability towards aggregation.
本文介绍了新型主客体功能化聚合物的合成，并通过小角 X 射线散射（SAXS）对所得纳米粒子进行了结构表征。通过点击反应铜（I）催化的叠氮化物-炔烃环加成（CuAAC）将单-6-去氧-单-6-叠氮基-β-CD（NβCD）接枝到炔基功能化的普鲁兰上，并通过相同的策略制备了金刚烷修饰的葡聚糖。使用核磁共振（NMR）光谱、凝胶过滤色谱（GFC）、等温热滴定法（ITC）和 SAXS 对聚合物进行了表征。纳米粒子是通过定义良好的β-CD-普鲁兰和金刚烷修饰的葡聚糖之间的主客体相互作用形成的。使用动态光散射（DLS）和 SAXS 进行了表征，结果表明粒径在亚 100nm 范围内的球形粒子。这些研究揭示了分子结构和主客体比例对关键性质（如粒径、粒径分布、孔隙率和抗聚集稳定性）的重要性。
