Glick R E, Triemer R E, Zilinskas B A
J Cell Sci. 1986 Feb;80:57-73. doi: 10.1242/jcs.80.1.57.
Thylakoid membranes of the thermophilic cyanobacterium Phormidium laminosum have been fractionated into photosystem II and photosystem I particles. These fractions have been characterized by their partial electron transport activities, and biochemical and spectral properties. Exoplasmic fracture face and protoplasmic fracture face particles in the unfractionated thylakoid membranes were shown to correspond in size to particles in freeze-fractured photosystem II and photosystem I fractions, respectively. Differences between the histograms of the thylakoid membrane protoplasmic fracture face particles and the isolated photosystem I particles suggest that in addition to photosystem I complexes some of the particles on the thylakoid protoplasmic fracture face may be related to cytochrome b/f complexes, the hydrophobic component of the coupling factor, or respiratory complexes.
嗜热蓝藻层状席藻的类囊体膜已被分离成光系统II和光系统I颗粒。这些组分通过其部分电子传递活性、生化和光谱特性进行了表征。未分级的类囊体膜中的细胞外断裂面颗粒和原生质断裂面颗粒的大小分别与冷冻断裂的光系统II和光系统I组分中的颗粒相对应。类囊体膜原生质断裂面颗粒和分离的光系统I颗粒的直方图之间的差异表明，除了光系统I复合物外，类囊体原生质断裂面上的一些颗粒可能与细胞色素b/f复合物、偶联因子的疏水成分或呼吸复合物有关。
