Staehelin L A, DeWit M
J Cell Biochem. 1984;24(3):261-9. doi: 10.1002/jcb.240240307.
Freeze-fracture electron microscopy has revealed that different size classes of intramembrane particles of chloroplast membranes are nonrandomly distributed between appressed grana and nonappressed stroma membrane regions. It is now generally assumed that thylakoid membranes contain five major functional complexes, each of which can give rise to an intramembrane particle of a defined size. These are the photosystem II complex, the photosystem I complex, the cytochrome f/b6 complex, the chlorophyll a/b light-harvesting complex, and the CF0 -CF1 ATP synthetase complex. By mapping the distribution of the different categories of intramembrane particles, information on the lateral organization of functional membrane units of thylakoid membranes can be determined. In this review, we present a brief summary of the evidence supporting the correlation of specific categories of intramembrane particles with known biochemical entities. In addition, we discuss studies showing that ions and phosphorylation of the membrane adhesion factor, the chlorophyll a/b light-harvesting complex, can affect the lateral organization of chloroplast membrane components and thereby regulate membrane function.
冷冻蚀刻电子显微镜显示，叶绿体膜中不同大小类别的膜内颗粒在紧密堆积的基粒和非紧密堆积的基质膜区域之间呈非随机分布。现在普遍认为，类囊体膜包含五种主要功能复合物，每种复合物都能产生特定大小的膜内颗粒。它们是光系统II复合物、光系统I复合物、细胞色素f/b6复合物、叶绿素a/b捕光复合物和CF0 -CF1 ATP合成酶复合物。通过绘制不同类别的膜内颗粒的分布图，可以确定类囊体膜功能膜单元的侧向组织信息。在这篇综述中，我们简要总结了支持特定类别的膜内颗粒与已知生化实体相关性的证据。此外，我们还讨论了一些研究，这些研究表明离子和膜粘附因子叶绿素a/b捕光复合物的磷酸化可以影响叶绿体膜成分的侧向组织，从而调节膜功能。
