Nilsson F, Simpson D J, Stewart A C, Andersson B
Department of Biochemistry, Arrhenius Laboratories, University of Stockholm, Sweden.
Arch Biochem Biophys. 1988 Sep;265(2):321-8. doi: 10.1016/0003-9861(88)90134-8.
A method has been designed to prepare inside-out thylakoid vesicles from a cyanobacterial species (Phormidium laminosum). Everted thylakoid vesicles could be generated by Yeda press treatment after induced membrane pairing. Membrane pairing was induced either by addition of high concentrations of Mg2+ ions or by lowering the pH of the fragmentation media. The inside-out vesicles were isolated by aqueous polymer two-phase partition. The membrane orientation was determined by proton translocation studies and freeze-fracture electron microscopy.
