Ishikawa Shu, Ueno Shohei, Mitsui Mai, Matsumura Yoshinobu, Hatsuoka Tetsuro
Engineering Division, Kajima Corporation.
Department of Life Science & Biotechnology, Kansai University.
Biocontrol Sci. 2019;24(1):57-65. doi: 10.4265/bio.24.57.
Effective spatial disinfection systems are required for human health care, public hygiene, and food and medicine manufacturing. Although some aerosolized disinfectants were already applied to its purpose, accurate evaluation systems were under constructed. In this study, the spatial sporicidal activity of aerosolized hypochlorite solution (AHS) to dormant cells, Bacillus subtilis spores, was evaluated by an originally designed chamber system. In the test-chamber, AHS was supplied and existed as micro-droplets, and environmental relative humidity (RH) could be controlled. Available chlorine (AC) exposure was also controlled with appropriate AC loading but was influenced by the acidity of AHS. Our results indicated that inactivation of spore was depend on AC exposure amount and time. On the other hand, unsaturated environmental RH markedly decreased spore inactivation. This study indicated that our test-chamber system can provide reproducible test data under a homogeneous air condition, and, thereby, that the data obtained by the chamber system should contribute to predicting the AC-required dose to disinfect a whole building.
有效的空间消毒系统对于人类医疗保健、公共卫生以及食品和药品制造至关重要。尽管一些雾化消毒剂已用于此目的，但准确的评估系统仍在构建中。在本研究中，通过一个专门设计的腔室系统评估了雾化次氯酸盐溶液（AHS）对休眠细胞枯草芽孢杆菌孢子的空间杀孢子活性。在测试腔室中，供应AHS并以微滴形式存在，环境相对湿度（RH）可以控制。有效氯（AC）暴露也通过适当的AC负载进行控制，但受AHS酸度的影响。我们的结果表明，孢子的失活取决于AC暴露量和时间。另一方面，不饱和环境RH显著降低孢子失活。本研究表明，我们的测试腔室系统可以在均匀的空气条件下提供可重复的测试数据，因此，通过腔室系统获得的数据应有助于预测对整个建筑物进行消毒所需的AC剂量。
