Zhao Yunpeng, Zhao Shukang, Chen Yingjie, Dong Xiaopeng, Peng Chuanliang, Sun Qifeng, Shan Lei, Wang Zhendan, Zhao Xiaogang
Department of Thoracic Surgery, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
Department of Thoracic Surgery, Shandong Cancer Hospital and Institute (HL), Jinan, Shandong 250177, P.R. China.
Oncol Lett. 2019 Apr;17(4):3817-3825. doi: 10.3892/ol.2019.10017. Epub 2019 Feb 6.
The clinical significance of circulating tumor cells (CTCs) in patients with esophageal squamous cell carcinoma (ESCC) who have undergone radical surgery was investigated. A novel confirmation method for identifying CTCs or circulating tumor microemboli (CTM) in ESCC was also investigated. Blood samples from 55 patients with ESCC were collected 1-3 days prior to surgery and 7 days post-surgery. All patients underwent curative thoracic esophagectomy and lymphadenectomy. Blood samples from 20 healthy volunteers were obtained as controls. Isolation by size of epithelial tumor cells (ISET) was performed also. The overall CTC detection rate was 52.7% preoperatively and 49.1% postoperatively. The presence of CTCs correlated with the Tumor-Node-Metastasis stage and the Log odds of positive lymph nodes. No significant difference in perioperative CTC transformation was discovered between the thoracoscopic and laparoscopic approach, and the open approach. The P40+/cluster of differentiation (CD)45- phenotype was confirmed in the CTCs and CTM. ISET appeared to have high sensitivity for detecting CTCs within ESCC patients. Immunofluorescence staining for CD45 and P40 was a specific, accurate and convenient method for confirming the presence of CTCs or CTM in patients with ESCC, and is strongly recommended as a supplement to morphological analysis.
研究了接受根治性手术的食管鳞状细胞癌（ESCC）患者循环肿瘤细胞（CTC）的临床意义。还研究了一种用于鉴定ESCC中CTC或循环肿瘤微栓子（CTM）的新型确认方法。在术前1 - 3天和术后7天收集55例ESCC患者的血样。所有患者均接受了根治性胸段食管切除术和淋巴结清扫术。获取20名健康志愿者的血样作为对照。还进行了上皮肿瘤细胞大小分离法（ISET）。术前CTC总体检测率为52.7%，术后为49.1%。CTC的存在与肿瘤-淋巴结-转移分期及阳性淋巴结的对数几率相关。在胸腔镜、腹腔镜和开放手术入路之间，未发现围手术期CTC转化有显著差异。在CTC和CTM中证实了P40⁺/分化簇（CD）45⁻表型。ISET似乎对检测ESCC患者体内的CTC具有高灵敏度。CD45和P40的免疫荧光染色是确认ESCC患者中CTC或CTM存在的一种特异性、准确且便捷的方法，强烈推荐作为形态学分析的补充方法。
