Morris Joshua D, Payne Christine K
School of Science and Technology, Georgia Gwinnett College, Lawrenceville, Georgia 30043, USA.
Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina 27708, USA; email:
Annu Rev Phys Chem. 2019 Jun 14;70:199-218. doi: 10.1146/annurev-physchem-042018-052527. Epub 2019 Mar 18.
Understanding the cellular basis of human health and disease requires the spatial resolution of microscopy and the molecular-level details provided by spectroscopy. This review highlights imaging methods at the intersection of microscopy and spectroscopy with applications in cell biology. Imaging methods are divided into three broad categories: fluorescence microscopy, label-free approaches, and imaging tools that can be applied to multiple imaging modalities. Just as these imaging methods allow researchers to address new biological questions, progress in biological sciences will drive the development of new imaging methods. We highlight four topics in cell biology that illustrate the need for new imaging tools: nanoparticle-cell interactions, intracellular redox chemistry, neuroscience, and the increasing use of spheroids and organoids. Overall, our goal is to provide a brief overview of individual imaging methods and highlight recent advances in the use of microscopy for cell biology.
了解人类健康与疾病的细胞基础需要显微镜的空间分辨率以及光谱学提供的分子水平细节。本综述重点介绍了显微镜学与光谱学交叉领域的成像方法及其在细胞生物学中的应用。成像方法分为三大类：荧光显微镜、无标记方法以及可应用于多种成像模式的成像工具。正如这些成像方法使研究人员能够解决新的生物学问题一样，生物科学的进展也将推动新成像方法的发展。我们重点介绍细胞生物学中的四个主题，以说明对新成像工具的需求：纳米颗粒与细胞的相互作用、细胞内氧化还原化学、神经科学以及球体和类器官的日益广泛应用。总体而言，我们的目标是简要概述各种成像方法，并突出显微镜在细胞生物学应用中的最新进展。
